RRC ID 40733
著者 Park HK, Han JH, Kim TS, Joung Y, Cho SH, Kwon SW, Kim SB.
タイトル Sphingomonas aeria sp. nov. from indoor air of a pharmaceutical environment.
ジャーナル Antonie Van Leeuwenhoek
Abstract A proteobacterial strain designated R1-3(T) was isolated from indoor air of a pharmaceutical environment. Cells were Gram-stain-negative, oxidase- and catalase-positive, aerobic, motile and rod-shaped. Strain R1-3(T) grew optimally at pH 7, 30 °C and in 0-2 % NaCl on R2A agar. The 16S rRNA gene sequence analysis indicated that strain R1-3(T) belongs to the genus Sphingomonas, and is closely related to Sphingomonas paucimobilis ATCC 29837(T) (98.4 % sequence similarity). However, the DNA-DNA relatedness between the two strains was 43 ± 5 % (reciprocal = 37 ± 3 %), which was well below the suggested level for species distinction. Sphingomonas yabuuchiae GTC868(T) (97.7 % 16S rRNA gene sequence similarity) and Sphingomonas pseudosanguinis G1-2(T) (97.6 %) were also found as distantly related taxa. Strain R1-3(T) was sensitive to most of the tested antibiotics except for erythromycin and streptomycin. The major fatty acid was a summed feature consisting of C18:1 ω7c and/or C18:1 ω6c, and minor proportions of C14:0 2-OH, C16:0 and a summed feature consisting of C16:1 ω7c and/or C16:1 ω6c were also present. The DNA G + C content was 67.2 ± 1.0 mol%. The major polyamines were sym-homospermidine and spermidine. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, and minor amounts of a sphingoglycolipid, a phospholipid, an aminoglycolipid and an unidentified lipid were also present. The phenotypic, phylogenetic and chemotaxonomic data not only supported the affiliation of strain R1-3(T) to the genus Sphingomonas, but also distinguished R1-3(T) from related species. On the basis of physiological, chemotaxonomic and phylogenetic evidences, strain R1-3(T) clearly merits recognition as a novel species of Sphingomonas, for which the name Sphingomonas aeria sp. nov. is proposed. The type strain is R1-3(T) (= KCTC 42061(T) = JCM 19859(T)).
巻・号 107(1)
ページ 47-53
公開日 2015-1-1
DOI 10.1007/s10482-014-0302-5
PMID 25318449
MeSH Aerobiosis Air Microbiology* Anti-Bacterial Agents / pharmacology Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Drug Industry Fatty Acids / analysis Locomotion Microbial Sensitivity Tests Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Spermidine / analysis Sphingomonas / classification* Sphingomonas / genetics Sphingomonas / isolation & purification* Sphingomonas / physiology Temperature
IF 2.674
引用数 5
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19859