| RRC ID |
40746
|
| 著者 |
Jojima T, Igari T, Moteki Y, Suda M, Yukawa H, Inui M.
|
| タイトル |
Promiscuous activity of (S,S)-butanediol dehydrogenase is responsible for glycerol production from 1,3-dihydroxyacetone in Corynebacterium glutamicum under oxygen-deprived conditions.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
Corynebacterium glutamicum can consume glucose to excrete glycerol under oxygen deprivation. Although glycerol synthesis from 1,3-dihydroxyacetone (DHA) has been speculated, no direct evidence has yet been provided in C. glutamicum. Enzymatic and genetic investigations here indicate that the glycerol is largely produced from DHA and, unexpectedly, the reaction is catalyzed by (S,S)-butanediol dehydrogenase (ButA) that inherently catalyzes the interconversion between S-acetoin and (S,S)-2,3-butanediol. Consequently, the following pathway for glycerol biosynthesis in the bacterium emerges: dihydroxyacetone phosphate is dephosphorylated by HdpA to DHA, which is subsequently reduced to glycerol by ButA. This study emphasizes the importance of promiscuous activity of the enzyme in vivo.
|
| 巻・号 |
99(3)
|
| ページ |
1427-33
|
| 公開日 |
2015-2-1
|
| DOI |
10.1007/s00253-014-6170-0
|
| PMID |
25363556
|
| MeSH |
Alcohol Oxidoreductases / genetics
Alcohol Oxidoreductases / metabolism*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Corynebacterium glutamicum / enzymology*
Corynebacterium glutamicum / genetics
Corynebacterium glutamicum / metabolism
Dihydroxyacetone / metabolism
Glycerol / metabolism*
Oxygen / metabolism*
|
| IF |
3.53
|
| 引用数 |
11
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 18229 |
