論文 - 詳細
| RRC ID | 40746 |
|---|---|
| 著者 | Jojima T, Igari T, Moteki Y, Suda M, Yukawa H, Inui M. |
| タイトル | Promiscuous activity of (S,S)-butanediol dehydrogenase is responsible for glycerol production from 1,3-dihydroxyacetone in Corynebacterium glutamicum under oxygen-deprived conditions. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
Corynebacterium glutamicum can consume glucose to excrete glycerol under oxygen deprivation. Although glycerol synthesis from 1,3-dihydroxyacetone (DHA) has been speculated, no direct evidence has yet been provided in C. glutamicum. Enzymatic and genetic investigations here indicate that the glycerol is largely produced from DHA and, unexpectedly, the reaction is catalyzed by (S,S)-butanediol dehydrogenase (ButA) that inherently catalyzes the interconversion between S-acetoin and (S,S)-2,3-butanediol. Consequently, the following pathway for glycerol biosynthesis in the bacterium emerges: dihydroxyacetone phosphate is dephosphorylated by HdpA to DHA, which is subsequently reduced to glycerol by ButA. This study emphasizes the importance of promiscuous activity of the enzyme in vivo. |
| 巻・号 | 99(3) |
| ページ | 1427-33 |
| 公開日 | 2015-2-1 |
| DOI | 10.1007/s00253-014-6170-0 |
| PMID | 25363556 |
| MeSH | Alcohol Oxidoreductases / genetics Alcohol Oxidoreductases / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Corynebacterium glutamicum / enzymology* Corynebacterium glutamicum / genetics Corynebacterium glutamicum / metabolism Dihydroxyacetone / metabolism Glycerol / metabolism* Oxygen / metabolism* |
| IF | 3.53 |
| 引用数 | 11 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 18229 |