| RRC ID |
40747
|
| 著者 |
Wang LY, Wang TS, Chen SF.
|
| タイトル |
Cohnella capsici sp. nov., a novel nitrogen-fixing species isolated from Capsicum annuum rhizosphere soil, and emended description of Cohnella plantaginis.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
A novel bacterial strain designated YN-59(T) was isolated from Capsicum annuum rhizosphere soil in China. The isolate was found to be aerobic, Gram-positive, rod-shaped and to form ellipsoidal or oval spores positioned centrally in swollen sporangia. On the basis of 16S rRNA gene sequence analysis, the isolated strain YN-59 was determined to be related to members of genus Cohnella. High levels of 16S rRNA gene sequence similarity were found between strain YN-59 and Cohnella plantaginis DSM 25424(T) (98.5 %) and Cohnella ginsengisoli DSM18997(T) (97.3 %); the 16S rRNA gene sequence similarities between strain YN-59 and the other strains recognized members of the genus Cohnella were below 97 %. The DNA-DNA hybridization values of strain YN-59 with C. plantaginis DSM 25424(T) and C. ginsengisoli DSM18997(T) were 44.2 ± 8.4 and 28.8 ± 5.8 %, respectively. The DNA G + C content of strain YN-59(T) was determined to be 59.32 mol %. The major isoprenoid quinone was identified as MK-7 and the predominant fatty acids as anteiso-C15:0 (45.32 %), iso-C16:0 (19.19 %), iso-C15:0 (9.65 %) and C16:0 (8.91 %). The polar lipids of strain YN-59(T) were found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol; several unidentified phospholipids were also detected. The diagnostic diamino acid in the cell wall was identified as meso-diaminopimelic. On the basis of its phenotypic and genotypic characteristics and levels of DNA-DNA hybridization, strain YN-59(T) is considered to represent a novel species of the genus Cohnella, for which the name Cohnella capsici sp. nov. (type strain YN-59(T) = CGMCC 1.12046(T) = JCM 19168(T)) is proposed.
|
| 巻・号 |
107(1)
|
| ページ |
133-9
|
| 公開日 |
2015-1-1
|
| DOI |
10.1007/s10482-014-0310-5
|
| PMID |
25367338
|
| MeSH |
Aerobiosis
Bacillales / classification*
Bacillales / genetics
Bacillales / isolation & purification*
Bacillales / physiology
Bacterial Typing Techniques
Base Composition
Capsicum*
China
Cluster Analysis
Cytosol / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Molecular Sequence Data
Nitrogen Fixation*
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Rhizosphere
Sequence Analysis, DNA
Soil Microbiology*
|
| IF |
2.674
|
| 引用数 |
5
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 19168
JCM 11465 |
