論文 - 詳細
| RRC ID | 40748 |
|---|---|
| 著者 | Han D, Cui HL. |
| タイトル | Halorubrum laminariae sp. nov., isolated from the brine of salted brown alga Laminaria. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
Two halophilic archaeal strains, R60(T) and R61, were isolated from the brine of salted brown alga Laminaria. Cells of the two strains were observed to be rod-shaped, stain Gram-negative and to lyse in distilled water. Strain R60(T) was found to contain gas vacuoles and to produce pink-pigmented colonies, while strain R61 lacked gas vacuoles and produces red-pigmented colonies. Both strains were found to be able to grow at 20-50 °C (optimum 30 °C), at 1.7-4.8 M NaCl (optimum 2.6-3.1 M NaCl), at 0-1.0 M MgCl2 (optimum 0.005-0.1 M MgCl2) and at pH 6.0-9.5 (optimum pH 7.0). The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to a sulfated mannosyl glucosyl diether produced by Halorubrum members of the Halobacteriaceae. The 16S rRNA gene sequences of the two strains were 99.9 % identical, showing 94.6-98.0 % similarity to those of members of the genus Halorubrum. The EF-2 gene similarity between strains R60(T) and R60 was 100 % and showed 84.6-94.5 % similarity to those of members of the genus Halorubrum. The DNA G+C contents of the two strains were determined to be 63.0 mol %. The DNA-DNA hybridization value between strain R60(T) and strain R61 was 92 % and the two strains showed low DNA-DNA relatedness with the most related members of Halorubrum. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain R60(T) (= CGMCC 1.12689(T) = JCM 30040(T)) and strain R61 (= CGMCC 1.12696) represent a novel species of the genus Halorubrum, for which the name Halorubrum laminariae sp. nov. is proposed. |
| 巻・号 | 107(1) |
| ページ | 217-23 |
| 公開日 | 2015-1-1 |
| DOI | 10.1007/s10482-014-0319-9 |
| PMID | 25367341 |
| MeSH | Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Archaeal / chemistry DNA, Archaeal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Environmental Microbiology* Glycolipids / analysis Halorubrum / classification* Halorubrum / genetics Halorubrum / isolation & purification* Halorubrum / physiology Hydrogen-Ion Concentration Molecular Sequence Data Nucleic Acid Hybridization Peptide Elongation Factor 2 / genetics Phaeophyceae Phospholipids / analysis Phylogeny Pigments, Biological / analysis RNA, Ribosomal, 16S / genetics Salts Sequence Analysis, DNA Sodium Chloride / metabolism Temperature |
| IF | 2.674 |
| 引用数 | 11 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 30040 |