論文 - 詳細
| RRC ID | 40774 |
|---|---|
| 著者 | Jojima T, Noburyu R, Sasaki M, Tajima T, Suda M, Yukawa H, Inui M. |
| タイトル | Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
Recombinant Corynebacterium glutamicum harboring genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) can produce ethanol under oxygen deprivation. We investigated the effects of elevating the expression levels of glycolytic genes, as well as pdc and adhB, on ethanol production. Overexpression of four glycolytic genes (pgi, pfkA, gapA, and pyk) in C. glutamicum significantly increased the rate of ethanol production. Overexpression of tpi, encoding triosephosphate isomerase, further enhanced productivity. Elevated expression of pdc and adhB increased ethanol yield, but not the rate of production. Fed-batch fermentation using an optimized strain resulted in ethanol production of 119 g/L from 245 g/L glucose with a yield of 95% of the theoretical maximum. Further metabolic engineering, including integration of the genes for xylose and arabinose metabolism, enabled consumption of glucose, xylose, and arabinose, and ethanol production (83 g/L) at a yield of 90 %. This study demonstrated that C. glutamicum has significant potential for the production of cellulosic ethanol. |
| 巻・号 | 99(3) |
| ページ | 1165-72 |
| 公開日 | 2015-2-1 |
| DOI | 10.1007/s00253-014-6223-4 |
| PMID | 25421564 |
| MeSH | Batch Cell Culture Techniques Corynebacterium glutamicum / genetics* Corynebacterium glutamicum / metabolism* Ethanol / metabolism* Gene Expression Genes, Bacterial Metabolic Engineering* Metabolic Networks and Pathways / genetics |
| IF | 3.53 |
| 引用数 | 39 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 18229 |