論文 - 詳細
| RRC ID | 40782 |
|---|---|
| 著者 | Satomura T, Ishikura M, Koyanagi T, Sakuraba H, Ohshima T, Suye S. |
| タイトル | Dye-linked D-amino acid dehydrogenase from the thermophilic bacterium Rhodothermus marinus JCM9785: characteristics and role in trans-4-hydroxy-L-proline catabolism. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
A gene from the thermophilic Gram-negative bacterium Rhodothermus marinus JCM9785, encoding a dye-linked D-amino acid dehydrogenase homologue, was overexpressed in Escherichia coli, and its product was purified and characterized. The expressed enzyme was a highly thermostable dye-linked D-amino acid dehydrogenase that retained more than 80% of its activity after incubation for 10 min at up to 70 °C. When enzyme-catalyzed dehydrogenation of several D-amino acids was carried out using 2,6-dichloroindophenol as the electron acceptor, D-phenylalanine was the most preferable substrate among the D-amino acids tested. Immediately upstream of the dye-linked D-amino acid dehydrogenase gene (dadh) was a gene encoding a 4-hydroxyproline 2-epimerase homologue (hypE). That gene was successfully expressed in E. coli, and the gene product exhibited strong 4-hydroxyproline 2-epimerase activity. Reverse transcription PCR and quantitative real-time PCR showed that the six genes containing the dadh and hypE genes were arranged in an operon and were required for catabolism of trans-4-hydroxy-L-proline in R. marinus. This is the first description of a dye-linked D-amino acid dehydrogenase (Dye-DADH) with broad substrate specificity involved in trans-4-hydroxy-L-proline catabolism. |
| 巻・号 | 99(10) |
| ページ | 4265-75 |
| 公開日 | 2015-5-1 |
| DOI | 10.1007/s00253-014-6263-9 |
| PMID | 25472442 |
| MeSH | Amino Acid Oxidoreductases / chemistry Amino Acid Oxidoreductases / genetics Amino Acid Oxidoreductases / metabolism* Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism* Enzyme Stability Escherichia coli / genetics Escherichia coli / metabolism Hydrogen-Ion Concentration Isomerism Kinetics Proline / chemistry Proline / metabolism* Rhodothermus / chemistry Rhodothermus / enzymology* Rhodothermus / genetics Substrate Specificity |
| IF | 3.53 |
| 引用数 | 5 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 9785 |