RRC ID 40784
Author Lee JJ, Srinivasan S, Lim S, Joe M, Im S, Kim MK.
Title Deinococcus puniceus sp. nov., a bacterium isolated from soil-irradiated gamma radiation.
Journal Curr. Microbiol.
Abstract A Gram-positive, coccus-shaped, crimson-color-pigmented bacterium was isolated from soil irradiated with 5 kGy gamma radiation and was designated strain DY1(T). Cells showed growth at 10-30 °C and pH 7-11 and were oxidase-negative and catalase-positive. Phylogenetic analyses of the 16S rRNA gene showed that the strain DY1(T) belonged to the genus Deinococcus with sequence similarities to Deinococcus aquatilis CCUG 53370(T) (96.2 %) and Deinococcus navajonensis KR-114(T) (94.1 %). Strain DY1(T) showed low level of DNA relatedness with D. aquatilis CCUG 53370(T) (41.3 ± 3.9 %). The DNA G + C content of DY1(T) was 58.7 mol%. Predominant fatty acids were summed feature 3 (C16:1 ω7c/ω6c), C16:0, and C17:0. The major amino acids were D-alanine, L-glutamic acid, glycine, and L-ornithine in the peptidoglycan. The major polar lipids were unknown phosphoglycolipids (PGL). Strain DY1(T) has resistance to gamma radiation and was found to be a novel species. Therefore, the strain was designated as DY1(T) (=KCTC 33027(T) = JCM 18576(T)), and the name Deinococcus puniceus sp. nov. is herein proposed.
Volume 70(4)
Pages 464-9
Published 2015-4
DOI 10.1007/s00284-014-0748-8
PMID 25477066
MeSH Amino Acids / analysis Bacterial Typing Techniques Base Composition Cell Wall / chemistry Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Deinococcus / classification* Deinococcus / genetics Deinococcus / isolation & purification* Deinococcus / physiology Fatty Acids / analysis Gamma Rays Glycolipids / analysis Hydrogen-Ion Concentration Molecular Sequence Data Nucleic Acid Hybridization Peptidoglycan / analysis Phospholipids / analysis Phylogeny Pigments, Biological / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Temperature
IF 1.373
Times Cited 5
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 18576