| RRC ID |
40788
|
| 著者 |
Kawamura Y, Kuwabara S, Kania SA, Kato H, Hamagishi M, Fujiwara N, Sato T, Tomida J, Tanaka K, Bemis DA.
|
| タイトル |
Porphyromonas pogonae sp. nov., an anaerobic but low concentration oxygen adapted coccobacillus isolated from lizards (Pogona vitticeps) or human clinical specimens, and emended description of the genus Porphyromonas Shah and Collins 1988.
|
| ジャーナル |
Syst Appl Microbiol
|
| Abstract |
During the process of identifying a Gram-negative coccobacillus isolated from a human clinical specimen, we found that the isolate's 16S rRNA gene had very close sequence identity with that of a variant Porphyromonas isolated from polymicrobial infections in the central bearded dragon, a species of lizard [2]. The 16S rRNA gene sequences of the human isolate and of six isolates from lizards were nearly identical (99.9-100%). Phylogenetic analysis placed all of these isolates in a single phylogenetic cluster well separated from other species in the genus Porphyromonas. The closest species was Porphyromonas catoniae with 90.7-90.9% sequence identity, although there was less than 6% DNA similarity between the P. catoniae type strain and our representative isolates from lizards (PAGU 1787(T)) and human (PAGU 1776). These isolates could grow under anaerobic or microaerobic conditions (6% O2 atmosphere). The isolates were positive for catalase and very strong β-hemolytic activity, but did not show black or brown pigmentation. Biochemically, the isolates could be differentiated from closely related species by pyroglutamic acid arylamidase and glycine arylamidase activity, and some others. The fermentation products mainly included succinic acid and propionic acid. The major fatty acids detected in cells of the isolates were iso-C15:0, anteiso-C15:0, and 3OH-iso-C17:0. The G+C content was 43.0 ± 0.62 mol%. The species name Porphyromonas pogonae sp. nov. is proposed for these isolates with the type strain of PAGU 1787(T) (=MI 10-1288(T)=JCM 19732(T)=ATCC BAA-2643(T)).
|
| 巻・号 |
38(2)
|
| ページ |
104-9
|
| 公開日 |
2015-3-1
|
| DOI |
10.1016/j.syapm.2014.11.004
|
| PII |
S0723-2020(14)00176-3
|
| PMID |
25481042
|
| MeSH |
Anaerobiosis
Animals
Bacterial Typing Techniques
Bacteroidaceae Infections / microbiology*
Base Composition
Catalase / analysis
Cluster Analysis
Cytosol / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Hemolysin Proteins / analysis
Humans
Lizards / microbiology*
Molecular Sequence Data
Oxygen / toxicity
Phylogeny
Pigments, Biological / metabolism
Porphyromonas / classification*
Porphyromonas / genetics
Porphyromonas / isolation & purification*
Porphyromonas / physiology
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
3.224
|
| 引用数 |
10
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 6326
JCM 13863
JCM 13865
JCM 12257
JCM 19732 |
