論文 - 詳細
| RRC ID | 40806 |
|---|---|
| 著者 | Morohoshi T, Tominaga Y, Someya N, Ikeda T. |
| タイトル | Characterization of a novel thermostable N-acylhomoserine lactonase from the thermophilic bacterium Thermaerobacter marianensis. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Thermaerobacter marianensis is an extremely thermophilic bacterium, which was isolated from the Mariana Trench, with an optimal growth temperature of approximately 75 °C. N-Acylhomoserine lactone (AHL) is a quorum-sensing signal molecule used by many gram-negative bacteria. Here, we report the identification of an AHL-degrading gene homolog (designated aiiT) in the genome of T. marianensis JCM 10246. AiiT has 59.7%, 21.2%, and 11.2% identity to AhlS from Solibacillus silvestris, AiiA from Bacillus cereus, and AidC from Chryseobacterium sp., respectively. Homologs of aiiT were also found in Thermaerobacter nagasakiensis, T. composti, and T. subterraneus. A purified AiiT-maltose binding fusion showed high AHL-degrading activity against N-hexanoyl-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and N-decanoyl-L-homoserine lactone at temperatures ranging from 40 to 80 °C. HPLC analysis revealed that AiiT functions as an AHL-lactonase that catalyzes AHL ring opening by hydrolyzing lactones. AiiT displayed maximal activity at high temperatures (60-80 °C) and showed higher thermostability than other AHL lactonases. |
| 巻・号 | 120(1) |
| ページ | 1-5 |
| 公開日 | 2015-7-1 |
| DOI | 10.1016/j.jbiosc.2014.11.014 |
| PII | S1389-1723(14)00453-8 |
| PMID | 25529553 |
| MeSH | 4-Butyrolactone / analogs & derivatives* 4-Butyrolactone / chemistry 4-Butyrolactone / metabolism Bacillus / enzymology Bacillus / genetics Bacillus cereus Bacteria, Aerobic / enzymology* Bacteria, Aerobic / genetics Carboxylic Ester Hydrolases / metabolism* Enzyme Stability Homoserine / analogs & derivatives Homoserine / metabolism Hydrolysis Lactones / metabolism Planococcaceae / enzymology Planococcaceae / genetics Quorum Sensing Substrate Specificity Temperature* |
| IF | 2.366 |
| 引用数 | 13 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 10246 JCM 11223 JCM 15650 |