RRC ID 40812
Author Mokudai T, Kanno T, Niwano Y.
Title Postantifungal-like effect of sublethal treatment of Candida albicans with acid-electrolyzed water.
Journal Arch. Oral Biol.
Abstract OBJECTIVE:Acid-electrolyzed water (AEW) has been applied to the treatment of oral candidiasis. We evaluated the postantifungal effect (PAFE)-like activity of AEW against Candida albicans under sublethal conditions by exposing C. albicans to dilute AEW.
DESIGN:The growth of C. albicans after a short-term exposure to dilute AEW was evaluated in broth and on agar culture. The involvement of reactive oxygen species (ROS) in the PAFE was examined by flow cytometric analysis with hydroxyphenyl fluorescein (HPF) as a fluorescence probe.
RESULTS:The dilute AEW exerted PAFE-like activity against C. albicans. ROS were produced in the cells treated with AEW diluted 16 times or fewer. The increase in HPF fluorescence after treatment with dilute AEW was cancelled by dimethyl sulfoxide, a hydroxyl radical (OH) scavenger.
CONCLUSION:It would be expected that the ROS, especially OH, produced in the C. albicans cells treated with sublethal dilutions of AEW could exert PAFE-like activity against the fungal cells.
Volume 60(3)
Pages 479-87
Published 2015-3
DOI 10.1016/j.archoralbio.2014.12.008
PII S0003-9969(14)00320-3
PMID 25555249
MeSH Antifungal Agents / pharmacology Candida albicans / drug effects* Candidiasis, Oral / drug therapy* Cell Survival / drug effects Disinfectants / chemistry Disinfectants / pharmacology* Electrolysis Hydrogen Peroxide / chemistry Hydrogen Peroxide / pharmacology* Hydrogen-Ion Concentration Hypochlorous Acid / chemistry Hypochlorous Acid / pharmacology* Microbial Viability / drug effects Reactive Oxygen Species
IF 2.05
Times Cited 0
General Microbes JCM 1537