| RRC ID |
40817
|
| Author |
Nakayama M, Shimatani K, Ozawa T, Shigemune N, Tomiyama D, Yui K, Katsuki M, Ikeda K, Nonaka A, Miyamoto T.
|
| Title |
Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Catechins are a class of polyphenols and have high anti-bacterial activity against various microorganisms. Here, we report the mechanism for antibacterial activity of epigallocatechin gallate (EGCg) against Gram-positive bacteria Bacillus subtilis, which is highly sensitive to EGCg. Transmission electron microscope analysis revealed that deposits containing EGCg were found throughout the cell envelope from the outermost surface to the outer surface of cytoplasmic membrane. Aggregating forms of proteins and EGCg were identified as spots that disappeared or showed markedly decreased intensity after the treatment with EGCg compared to the control by two-dimensional electrophoresis. Among the identified proteins included 4 cell surface proteins, such as oligopeptide ABC transporter binding lipoprotein, glucose phosphotransferase system transporter protein, phosphate ABC transporter substrate-binding protein, and penicillin-binding protein 5. Observations of glucose uptake of cells and cell shape B. subtilis after the treatment with EGCg suggested that EGCg inhibits the major functions of these proteins, leading to growth inhibition of B. subtilis.
|
| Volume |
79(5)
|
| Pages |
845-54
|
| Published |
2015-1-1
|
| DOI |
10.1080/09168451.2014.993356
|
| PMID |
25559894
|
| MeSH |
Amino Acid Sequence
Anti-Bacterial Agents / pharmacology*
Bacillus subtilis / drug effects*
Bacillus subtilis / metabolism
Bacillus subtilis / physiology
Bacterial Proteins / antagonists & inhibitors
Bacterial Proteins / chemistry
Bacterial Proteins / metabolism*
Carrier Proteins / chemistry
Carrier Proteins / metabolism
Catechin / analogs & derivatives*
Catechin / chemistry
Catechin / metabolism
Catechin / pharmacology
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Glucose / metabolism
Lipoproteins / chemistry
Lipoproteins / metabolism
Molecular Docking Simulation
Molecular Sequence Data
Oxygen / metabolism
Spores, Bacterial / drug effects
|
| IF |
1.516
|
| Times Cited |
32
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 1465 |
