RRC ID 40891
著者 Romanenko LA, Tanaka N, Svetashev VI, Kalinovskaya NI, Mikhailov VV.
タイトル Rheinheimera japonica sp. nov., a novel bacterium with antimicrobial activity from seashore sediments of the Sea of Japan.
ジャーナル Arch Microbiol
Abstract Two gram-negative, aerobic, brown-pigmented, motile rod-shaped bacteria KMM 9512 and KMM 9513(T) were isolated from a sediment sample collected from the Sea of Japan seashore, Russia. On the basis of the 16S rRNA gene sequence analysis, novel strains KMM 9512 and KMM 9513(T) positioned within the genus Rheinheimera (class Gammaproteobacteria) as a separate subline adjacent to Rheinheimera baltica DSM 14885(T) sharing highest gene sequence similarities of 98.6-97.6 % to their closest phylogenetic relatives, Rheinheimera muenzenbergensis LMG 27269(T), R. baltica DSM 14885(T), Rheinheimera aquimaris JCM 14331(T), Rheinheimera nanhaiensis KACC 14030(T), and Rheinheimera pacifica KMM 1406(T). Strains KMM 9512 and KMM 9513(T) belong to the same separate genospecies on the basis of the 16S rRNA gene sequence similarity (99.8 %) and their DNA relatedness to each other (89 %) and to closely related Rheinheimera species (25-53 %). The major isoprenoid quinone was Q-8, polar lipids consisted of phosphatidylserine, phosphatidylglycerol, phosphatidylethanolamine, unknown aminolipids, unknown aminophospholipids, unknown phospholipids, and unknown lipids, and major fatty acid were C16:0, C16:1 ω7c, C17:1 ω8c, C12:0 3-OH followed by C17:0 and C18:1 ω7c in both strains. Strains KMM 9512 and KMM 9513(T) revealed a remarkable antagonistic activity toward a number of gram-positive and gram-negative microorganisms. On the basis of phylogenetic analysis, DNA-DNA hybridization results, and phenotypic differences, strains KMM 9512 and KMM 9513(T) are proposed to be classified as a novel species of the genus Rheinheimera, Rheinheimera japonica sp. nov. The type strain of this species is KMM 9513(T) = NRIC 0918(T).
巻・号 197(4)
ページ 613-20
公開日 2015-5-1
DOI 10.1007/s00203-015-1095-2
PMID 25702316
MeSH Anti-Bacterial Agents / metabolism* Anti-Bacterial Agents / pharmacology Base Sequence Chromatiaceae / classification Chromatiaceae / genetics Chromatiaceae / isolation & purification Chromatiaceae / metabolism* DNA, Bacterial / genetics Fatty Acids / analysis Geologic Sediments / microbiology* Gram-Negative Bacteria / drug effects* Gram-Positive Bacteria / drug effects* Japan Microbial Sensitivity Tests Molecular Sequence Data Nucleic Acid Hybridization Oceans and Seas Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 1.884
引用数 12
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 14331