論文 - 詳細
| RRC ID | 40905 |
|---|---|
| 著者 | Li ZS, Noda K, Fujita E, Manabe Y, Hirata T, Sugawara T. |
| タイトル | The green algal carotenoid siphonaxanthin inhibits adipogenesis in 3T3-L1 preadipocytes and the accumulation of lipids in white adipose tissue of KK-Ay mice. |
| ジャーナル | J Nutr |
| Abstract |
BACKGROUND:Siphonaxanthin, a xanthophyll present in green algae, has been shown to possess antiangiogenic and apoptosis-inducing activities. OBJECTIVE:We evaluated the antiobesity effects of siphonaxanthin by using a 3T3-L1 cell culture system and in diabetic KK-Ay mice. METHODS:3T3-L1 cells were differentiated with or without 5 μmol/L siphonaxanthin, and lipid accumulation and critical gene expressions for adipogenesis were examined. In vivo, 4-wk-old male KK-Ay mice were administered daily oral treatment of 1.3 mg siphonaxanthin for 6 wk and body weight, visceral fat weight, serum variables, and gene expressions involved in lipid metabolism were evaluated. RESULTS:Compared with the other carotenoids evaluated, siphonaxanthin potently inhibited adipocyte differentiation. Siphonaxanthin significantly suppressed lipid accumulation at noncytotoxic concentrations of 2.5 and 5 μmol/L by 29% and 43%, respectively. The effects of siphonaxanthin were largely limited to the early stages of adipogenesis. Siphonaxanthin significantly inhibited protein kinase B phosphorylation by 48% and 72% at 90 and 120 min, respectively. The expressions of key adipogenesis genes, including CCAAT/enhancer binding protein α (Cebpa), peroxisome proliferator activated receptor γ (Pparg), fatty acid binding protein 4 (Fabp4), and stearoyl coenzyme A desaturase 1 (Scd1), were elevated by 1.6- to 166-fold during adipogenesis. After 8 d of adipocyte differentiation, siphonaxanthin significantly lowered gene expression of Cebpa, Pparg, Fabp4, and Scd1 by 94%, 83%, 95%, and 90%, respectively. Moreover, oral administration of siphonaxanthin to KK-Ay mice significantly reduced the total weight of white adipose tissue (WAT) by 13%, especially the mesenteric WAT by 28%. Furthermore, siphonaxanthin administration reduced lipogenesis and enhanced fatty acid oxidation in adipose tissue. Siphonaxanthin was observed to highly accumulate in mesenteric WAT, and the accumulation in the mesenteric WAT was almost 2- and 3-fold that in epididymal (P = 0.14) and perirenal (P < 0.05) WAT, respectively. CONCLUSION:These results provide evidence that siphonaxanthin may effectively regulate adipogenesis in 3T3-L1 cells and diabetic KK-Ay mice. |
| 巻・号 | 145(3) |
| ページ | 490-8 |
| 公開日 | 2015-3-1 |
| DOI | 10.3945/jn.114.200931 |
| PII | jn.114.200931 |
| PMID | 25733464 |
| MeSH | 3T3-L1 Cells Adipocytes / drug effects* Adipogenesis / drug effects* Adipogenesis / genetics Adipose Tissue, White / drug effects* Adipose Tissue, White / metabolism Administration, Oral Animals Blood Glucose / metabolism CCAAT-Enhancer-Binding Proteins / genetics CCAAT-Enhancer-Binding Proteins / metabolism Cell Differentiation / drug effects Chlorophyta / chemistry* Cholesterol / blood Fatty Acid-Binding Proteins / genetics Fatty Acid-Binding Proteins / metabolism Lipid Metabolism / drug effects Male Mice PPAR gamma / genetics PPAR gamma / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Stearoyl-CoA Desaturase / genetics Stearoyl-CoA Desaturase / metabolism Triglycerides / blood Xanthophylls / pharmacology* |
| IF | 4.281 |
| 引用数 | 21 |
| WOS 分野 | NUTRITION & DIETETICS |
| リソース情報 | |
| 一般微生物 | JCM 13552 |