| RRC ID |
40916
|
| Author |
Kodani S, Komaki H, Suzuki M, Hemmi H, Ohnishi-Kameyama M.
|
| Title |
Isolation and structure determination of new siderophore albachelin from Amycolatopsis alba.
|
| Journal |
Biometals
|
| Abstract |
A new siderophore named albachelin was isolated from iron deficient culture of Amycolatopsis alba. The planar structure of albachelin was elucidated by the combination of ESI-MS/MS experiment and NMR spectroscopic analyses of the gallium (III) complex. The structure of albachelin was determined to be a linear peptide consisting of 6 mol of amino acids including 3 mol of serine, one mol each of N-α-acethyl-N-δ-hydroxy-N-δ-formylornithine, N-α-methyl-N-δ-hydroxyornithine, and cyclic N-hydroxyornithine. The stereochemistries of amino acids constituting albachelin were analyzed by applying modified Marfey method to the hydrolysate of albachelin. Based on bioinformatics, we deduced and discussed the possible biosynthetic gene cluster involved in albachelin biosynthesis from the genome sequence of A. alba. By prediction of substrates for adenylation domains, a non-ribosomal peptide biosynthetase gene (AMYAL_RS0130210) was proposed to be the main biosynthetic gene for albachelin biosynthesis. The related genes including transporter for siderophore were found near the NRPS gene as a gene cluster.
|
| Volume |
28(2)
|
| Pages |
381-9
|
| Published |
2015-4-1
|
| DOI |
10.1007/s10534-015-9842-z
|
| PMID |
25749409
|
| MeSH |
Actinomycetales / chemistry
Actinomycetales / genetics*
Actinomycetales / metabolism
Bacterial Proteins / genetics
Biosynthetic Pathways
Gallium / chemistry
Genes, Bacterial
Magnetic Resonance Spectroscopy
Molecular Structure
Multigene Family
Siderophores / biosynthesis
Siderophores / chemistry*
Siderophores / isolation & purification
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
|
| IF |
2.479
|
| Times Cited |
21
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 10030 |
