RRC ID 40921
著者 Iwamori M, Tanaka K, Adachi S, Aoki D, Nomura T.
タイトル Absence of lactobacilli containing glycolipids with the α-galactose epitope and the enhanced fucosylation of a receptor glycolipid GA1 in the digestive tracts of immune-deficient scid mice.
ジャーナル J Biochem
Abstract The Lactobacillus species in the digestive tracts of immune-deficient scid mice was distinct from that in control mice, i.e. Lactobacillus murinus in scid and L. johnsonii in control mice, according to their 16S-rRNA, indicating that a symbiotic relationship between lactobacilli and a host is established under pressure from the immune system. The caecal and colonal contents rich in L. murinus of scid mice were loose with a strong sour smell, resulting in diarrhoea, and those with L. johnsonii in control mice included abundant solid materials. Lactobacillus glycolipids were revealed to be recognized by the immune system, and by TLC-immunostaining, LacTetH-DG (Galα1-6Galα1-6Galα1-2Glcα1-3'DG) of L. johnsonii was detected in the stomach, caecum and colon of control mice, but not in those of scid ones, in which fucosylation of a receptor GA1 for L. johnsonii was enhanced more than 4-fold compared with in the control mice. Thus, structural modification of receptor glycolipids was revealed to occur in the process of establishment of a symbiotic relationship between lactobacilli and a host. LacTetH-DG was also immunogenic to human, because of the presence of natural antibodies against it, and the antibody binding to it was comparable to that of blood group- and species-related glycosphingolipids.
巻・号 158(1)
ページ 73-82
公開日 2015-7-1
DOI 10.1093/jb/mvv021
PII mvv021
PMID 25759397
MeSH Animals Antibodies / blood Antibodies / immunology Epitopes / chemistry Epitopes / immunology* Female Fucose / chemistry Fucose / immunology* Galactose / chemistry Galactose / immunology* Glycolipids / chemistry Glycolipids / deficiency Glycolipids / immunology* Humans Intestines / immunology Intestines / microbiology Lactobacillus / chemistry Lactobacillus / immunology* Mice Mice, Inbred C57BL Mice, SCID Receptors, Cell Surface / chemistry Receptors, Cell Surface / immunology*
IF 2.476
引用数 2
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 1022 JCM 7548 JCM 1112 JCM 2414 JCM 5707