論文 - 詳細
| RRC ID | 40923 |
|---|---|
| 著者 | Ferrario C, Milani C, Mancabelli L, Lugli GA, Turroni F, Duranti S, Mangifesta M, Viappiani A, Sinderen Dv, Ventura M. |
| タイトル | A genome-based identification approach for members of the genus Bifidobacterium. |
| ジャーナル | FEMS Microbiol Ecol |
| Abstract |
During recent years, the significant and increasing interest in novel bifidobacterial strains with health-promoting characteristics has catalyzed the development of methods for efficient and reliable identification of Bifidobacterium strains at (sub) species level. We developed an assay based on recently acquired bifidobacterial genomic data and involving 98 primer pairs, called the Bifidobacterium-ampliseq panel. This panel includes multiplex PCR primers that target both core and variable genes of the pangenome of this genus. Our results demonstrate that the employment of the Bifidobacterium-ampliseq panel allows rapid and specific identification of the so far recognized 48 (sub)species harboring the Bifidobacterium genus, and thus represents a cost- and time-effective bifidobacterial screening methodology. |
| 巻・号 | 91(3) |
| 公開日 | 2015-3-1 |
| DOI | 10.1093/femsec/fiv009 |
| PII | fiv009 |
| PMID | 25764568 |
| MeSH | Base Sequence Bifidobacterium / classification* Bifidobacterium / genetics Bifidobacterium / isolation & purification* DNA Primers / genetics DNA, Bacterial / genetics* Genome, Bacterial / genetics* Polymerase Chain Reaction / methods* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| IF | 3.675 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 7017 JCM 7019 JCM 1207 JCM 13495 |