Reference - Detail
| RRC ID | 40949 |
|---|---|
| Author | Carasi P, Racedo SM, Jacquot C, Romanin DE, Serradell MA, Urdaci MC. |
| Title | Impact of kefir derived Lactobacillus kefiri on the mucosal immune response and gut microbiota. |
| Journal | J Immunol Res |
| Abstract |
The evaluation of the impact of probiotics on host health could help to understand how they can be used in the prevention of diseases. On the basis of our previous studies and in vitro assays on PBMC and Caco-2 ccl20:luc reporter system presented in this work, the strain Lactobacillus kefiri CIDCA 8348 was selected and administrated to healthy Swiss mice daily for 21 days. The probiotic treatment increased IgA in feces and reduced expression of proinflammatory mediators in Peyer Patches and mesenteric lymph nodes, where it also increased IL-10. In ileum IL-10, CXCL-1 and mucin 6 genes were upregulated; meanwhile in colon mucin 4 was induced whereas IFN-γ, GM-CSF, and IL-1β genes were downregulated. Moreover, ileum and colon explants showed the anti-inflammatory effect of L. kefiri since the LPS-induced increment of IL-6 and GM-CSF levels in control mice was significantly attenuated in L. kefiri treated mice. Regarding fecal microbiota, DGGE profiles allowed differentiation of experimental groups in two separated clusters. Quantitative PCR analysis of different bacterial groups revealed only significant changes in Lactobacillus population. In conclusion, L. kefiri is a good candidate to be used in gut inflammatory disorders. |
| Volume | 2015 |
| Pages | 361604 |
| Published | 2015-1-1 |
| DOI | 10.1155/2015/361604 |
| PMID | 25811034 |
| PMC | PMC4355334 |
| MeSH | Animals Cell Line Cultured Milk Products / microbiology* Cytokines / biosynthesis Feces / microbiology Gastrointestinal Microbiome / immunology* Gene Expression Gene Expression Regulation Genes, Reporter Humans Immunity, Mucosal* / genetics Immunity, Mucosal* / immunology Immunoglobulin A / immunology Intestinal Mucosa / immunology Intestinal Mucosa / metabolism Intestinal Mucosa / microbiology Lactobacillus / immunology* Leukocytes, Mononuclear / immunology Leukocytes, Mononuclear / metabolism Lymphocyte Subsets / immunology Lymphocyte Subsets / metabolism Male Mice |
| IF | 3.327 |
| Times Cited | 37 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 24 |
| Altmetric score changes over past 6months | 3.2 |
| Resource | |
| General Microbes | JCM 5818 |