RRC ID 40952
Author Okuno T, Tani K, Yamaguchi N, Nasu M.
Title Expression of gyrB and 16S ribosomal RNA genes as indicators of growth and physiological activities of Legionella pneumophila.
Journal Biocontrol Sci
Abstract To determine whether the DNA gyrase (gyrB) and 16S ribosomal RNA (16S rRNA) genes can be used as indicators of the biological activities of Legionella pneumophila, the expression levels were estimated. The ratio of mRNA/DNA in gyrB was 0.7 in mid log phase and decreased drastically after the log phase. For 16S rRNA, the ratio was highest in mid log phase (7.0×10(3)), and the value that was about 10% of that in the log phase was maintained for six days. The rRNA may be vital in the resting or active but nonculturable cells that are not growing but physiologically active. The expression levels of gyrB mRNA and 16S rRNA can be used as indicators of the growth activity and the physiological activity of L. pneumophila, respectively. Therefore, by measurement of these indicators, we can evaluate the activities of Legionella cells in various environments.
Volume 20(1)
Pages 67-70
Published 2015-1-1
DOI 10.4265/bio.20.67
PMID 25817815
MeSH Bacteriological Techniques / methods* DNA Gyrase / analysis* DNA Gyrase / genetics Gene Expression* Legionella pneumophila / genetics Legionella pneumophila / physiology* RNA, Messenger / analysis* RNA, Messenger / genetics RNA, Ribosomal, 16S / analysis* RNA, Ribosomal, 16S / genetics
IF 0.833
Times Cited 2
General Microbes JCM 7571