RRC ID 40952
著者 Okuno T, Tani K, Yamaguchi N, Nasu M.
タイトル Expression of gyrB and 16S ribosomal RNA genes as indicators of growth and physiological activities of Legionella pneumophila.
ジャーナル Biocontrol Sci
Abstract To determine whether the DNA gyrase (gyrB) and 16S ribosomal RNA (16S rRNA) genes can be used as indicators of the biological activities of Legionella pneumophila, the expression levels were estimated. The ratio of mRNA/DNA in gyrB was 0.7 in mid log phase and decreased drastically after the log phase. For 16S rRNA, the ratio was highest in mid log phase (7.0×10(3)), and the value that was about 10% of that in the log phase was maintained for six days. The rRNA may be vital in the resting or active but nonculturable cells that are not growing but physiologically active. The expression levels of gyrB mRNA and 16S rRNA can be used as indicators of the growth activity and the physiological activity of L. pneumophila, respectively. Therefore, by measurement of these indicators, we can evaluate the activities of Legionella cells in various environments.
巻・号 20(1)
ページ 67-70
公開日 2015-1-1
DOI 10.4265/bio.20.67
PMID 25817815
MeSH Bacteriological Techniques / methods* DNA Gyrase / analysis* DNA Gyrase / genetics Gene Expression* Legionella pneumophila / genetics Legionella pneumophila / physiology* RNA, Messenger / analysis* RNA, Messenger / genetics RNA, Ribosomal, 16S / analysis* RNA, Ribosomal, 16S / genetics
IF 0.833
引用数 2
WOS 分野 BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
一般微生物 JCM 7571