RRC ID |
40996
|
著者 |
Maeda K, Spor A, Edel-Hermann V, Heraud C, Breuil MC, Bizouard F, Toyoda S, Yoshida N, Steinberg C, Philippot L.
|
タイトル |
N2O production, a widespread trait in fungi.
|
ジャーナル |
Sci Rep
|
Abstract |
N2O is a powerful greenhouse gas contributing both to global warming and ozone depletion. While fungi have been identified as a putative source of N2O, little is known about their production of this greenhouse gas. Here we investigated the N2O-producing ability of a collection of 207 fungal isolates. Seventy strains producing N2O in pure culture were identified. They were mostly species from the order Hypocreales order-particularly Fusarium oxysporum and Trichoderma spp.-and to a lesser extent species from the orders Eurotiales, Sordariales, and Chaetosphaeriales. The N2O (15)N site preference (SP) values of the fungal strains ranged from 15.8‰ to 36.7‰, and we observed a significant taxa effect, with Penicillium strains displaying lower SP values than the other fungal genera. Inoculation of 15 N2O-producing strains into pre-sterilized arable, forest and grassland soils confirmed the ability of the strains to produce N2O in soil with a significant strain-by-soil effect. The copper-containing nitrite reductase gene (nirK) was amplified from 45 N2O-producing strains, and its genetic variability showed a strong congruence with the ITS phylogeny, indicating vertical inheritance of this trait. Taken together, this comprehensive set of findings should enhance our knowledge of fungi as a source of N2O in the environment.
|
巻・号 |
5
|
ページ |
9697
|
公開日 |
2015-4-20
|
DOI |
10.1038/srep09697
|
PII |
srep09697
|
PMID |
25894103
|
PMC |
PMC4403702
|
MeSH |
Base Sequence
Biomass
Chromatography, Gas
DNA / analysis
Fungi / genetics
Fungi / isolation & purification
Fungi / metabolism*
Molecular Sequence Data
Nitrite Reductases / classification
Nitrite Reductases / genetics
Nitrogen Isotopes / chemistry
Nitrogen Oxides / analysis
Nitrogen Oxides / chemistry
Nitrogen Oxides / metabolism*
Phylogeny
Polymerase Chain Reaction
Sequence Analysis, DNA
Soil Microbiology
|
IF |
3.998
|
引用数 |
71
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 11502 |