RRC ID 40996
著者 Maeda K, Spor A, Edel-Hermann V, Heraud C, Breuil MC, Bizouard F, Toyoda S, Yoshida N, Steinberg C, Philippot L.
タイトル N2O production, a widespread trait in fungi.
ジャーナル Sci Rep
Abstract N2O is a powerful greenhouse gas contributing both to global warming and ozone depletion. While fungi have been identified as a putative source of N2O, little is known about their production of this greenhouse gas. Here we investigated the N2O-producing ability of a collection of 207 fungal isolates. Seventy strains producing N2O in pure culture were identified. They were mostly species from the order Hypocreales order-particularly Fusarium oxysporum and Trichoderma spp.-and to a lesser extent species from the orders Eurotiales, Sordariales, and Chaetosphaeriales. The N2O (15)N site preference (SP) values of the fungal strains ranged from 15.8‰ to 36.7‰, and we observed a significant taxa effect, with Penicillium strains displaying lower SP values than the other fungal genera. Inoculation of 15 N2O-producing strains into pre-sterilized arable, forest and grassland soils confirmed the ability of the strains to produce N2O in soil with a significant strain-by-soil effect. The copper-containing nitrite reductase gene (nirK) was amplified from 45 N2O-producing strains, and its genetic variability showed a strong congruence with the ITS phylogeny, indicating vertical inheritance of this trait. Taken together, this comprehensive set of findings should enhance our knowledge of fungi as a source of N2O in the environment.
巻・号 5
ページ 9697
公開日 2015-4-20
DOI 10.1038/srep09697
PII srep09697
PMID 25894103
PMC PMC4403702
MeSH Base Sequence Biomass Chromatography, Gas DNA / analysis Fungi / genetics Fungi / isolation & purification Fungi / metabolism* Molecular Sequence Data Nitrite Reductases / classification Nitrite Reductases / genetics Nitrogen Isotopes / chemistry Nitrogen Oxides / analysis Nitrogen Oxides / chemistry Nitrogen Oxides / metabolism* Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Soil Microbiology
IF 3.998
引用数 71
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 11502