| RRC ID |
41050
|
| 著者 |
Yanokura E, Oki K, Makino H, Modesto M, Pot B, Mattarelli P, Biavati B, Watanabe K.
|
| タイトル |
Subspeciation of Bifidobacterium longum by multilocus approaches and amplified fragment length polymorphism: Description of B. longum subsp. suillum subsp. nov., isolated from the faeces of piglets.
|
| ジャーナル |
Syst Appl Microbiol
|
| Abstract |
The species Bifidobacterium longum is currently divided into three subspecies, B. longum subsp. longum, B. longum subsp. infantis and B. longum subsp. suis. This classification was based on an assessment of accumulated information on the species' phenotypic and genotypic features. The three subspecies of B. longum were investigated using genotypic identification [amplified-fragment length polymorphism (AFLP), multilocus sequence analysis (MLSA) and multilocus sequence typing (MLST)]. By using the AFLP and the MLSA methods, we allocated 25 strains of B. longum into three major clusters corresponding to the three subspecies; the cluster comprising the strains of B. longum subsp. suis was further divided into two subclusters differentiable by the ability to produce urease. By using the MLST method, the 25 strains of B. longum were divided into eight groups: four major groups corresponding to the results obtained by AFLP and MLSA, plus four minor disparate groups. The results of AFLP, MLSA and MLST analyses were consistent and revealed a novel subspeciation of B. longum, which comprised three known subspecies and a novel subspecies of urease-negative B. longum, for which the name B. longum subsp. suillum subsp. nov. is proposed, with type strain Su 851(T)=DSM 28597(T)=JCM 19995(T).
|
| 巻・号 |
38(5)
|
| ページ |
305-14
|
| 公開日 |
2015-7-1
|
| DOI |
10.1016/j.syapm.2015.05.001
|
| PII |
S0723-2020(15)00073-9
|
| PMID |
26007614
|
| MeSH |
Amplified Fragment Length Polymorphism Analysis*
Animals
Animals, Newborn
Bifidobacterium / classification*
Bifidobacterium / genetics*
Bifidobacterium / isolation & purification
Cluster Analysis
Feces / microbiology*
Genotype*
Molecular Sequence Data
Multilocus Sequence Typing*
Sequence Analysis, DNA
Swine
|
| IF |
3.224
|
| 引用数 |
19
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1269
JCM 7139
JCM 19995 |
