| RRC ID |
41057
|
| Author |
Yoshida M, Yoshida-Takashima Y, Nunoura T, Takai K.
|
| Title |
Identification and genomic analysis of temperate Pseudomonas bacteriophage PstS-1 from the Japan trench at a depth of 7000 m.
|
| Journal |
Res Microbiol
|
| Abstract |
Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Trench in the Northwest Pacific Ocean. The genome size of PstS-1 was 48,666 bp; its genome displayed a 59.8% G + C content and a total of 79 coding sequences were identified in its genome. The PstS-1 phage belongs to the family Siphoviridae, but its genomic sequence and organization are distinct from those of any other well-known Siphoviridae phage. The mosaic genomic structure of PstS-1 suggests the occurrence of genetic exchange between distinct temperate phages in deep-sea Pseudomonas populations. The PstS-1 genome also harbors three distinct sequence regions corresponding to spacers within a single clustered regularly interspaced short palindromic repeat (CRISPR) locus in the rhizosphere-associated diazotrophic P. stutzeri A1501 genome. The extension of these spacers to the soil environment and the presence of many homologs of both the hadal deep-sea phage PstS-1 and terrestrial Pseudomonas phages suggest the early co-evolution of temperate phages and their host genus Pseudomonas prior to the divergence of their habitational and physiological adaptation.
|
| Volume |
166(9)
|
| Pages |
668-76
|
| Published |
2015-11-1
|
| DOI |
10.1016/j.resmic.2015.05.001
|
| PII |
S0923-2508(15)00076-5
|
| PMID |
26025640
|
| MeSH |
Genome, Viral
Genomics
Japan
Oceans and Seas
Phylogeny
Pseudomonas Phages / classification
Pseudomonas Phages / genetics*
Pseudomonas Phages / isolation & purification*
Pseudomonas Phages / ultrastructure
Pseudomonas stutzeri / virology*
Seawater / microbiology*
Seawater / virology
Sequence Analysis, DNA
Siphoviridae / genetics
Siphoviridae / isolation & purification*
|
| IF |
3.217
|
| Times Cited |
8
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 30558 |
