| RRC ID |
41068
|
| 著者 |
Ishibashi N, Seto H, Koga S, Zendo T, Sonomoto K.
|
| タイトル |
Identification of Lactococcus-Specific Bacteriocins Produced by Lactococcal Isolates, and the Discovery of a Novel Bacteriocin, Lactococcin Z.
|
| ジャーナル |
Probiotics Antimicrob Proteins
|
| Abstract |
Lactic acid bacteria that produce Lactococcus-specific bacteriocins were isolated and identified as Lactococcus lactis from fresh corn or lettuce. Among them, four isolates were identified as lactococcin Q producers. Seven isolates showed antimicrobial activity against a lactococcin Q producer, L. lactis QU 4, as well as against nisin Z and lacticin Q producers belonging to L. lactis. Strain QU 7 was selected as a standard strain and showed no cross-immunity to lactococcin Q or other lactococcal bacteriocins. The bacteriocin produced by strain QU 7 was purified in three chromatographic steps, and its molecular mass was determined to be 5041.35 Da. The amino acid sequence analysis revealed that it is a novel class IId bacteriocin, referred to as lactococcin Z. It consisted of 45 amino acid residues. The lczA gene encoding the prepeptide of lactococcin Z showed homology to lactococcins A, B, and M. Thus, this report demonstrates a new example of Lactococcus-specific bacteriocins.
|
| 巻・号 |
7(3)
|
| ページ |
222-31
|
| 公開日 |
2015-9-1
|
| DOI |
10.1007/s12602-015-9196-4
|
| PMID |
26093857
|
| MeSH |
Amino Acid Sequence
Bacteriocins / chemistry
Bacteriocins / isolation & purification*
Base Sequence
DNA, Bacterial / genetics
Genes, Bacterial
Lactococcus lactis / genetics
Lactococcus lactis / metabolism*
Molecular Sequence Data
Molecular Weight
Nisin / analogs & derivatives
Nisin / chemistry
Nisin / isolation & purification
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Zea mays / microbiology
|
| IF |
3.533
|
| 引用数 |
5
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 7638
JCM 2257
JCM 1465
JCM 2504
JCM 5885
JCM 5887
JCM 5803
JCM 5804
JCM 1180
JCM 5706
JCM 1157
JCM 6124
JCM 5802
JCM 5707 |
