論文 - 詳細
| RRC ID | 41090 |
|---|---|
| 著者 | Chung EJ, Park TS, Kim KH, Jeon CO, Lee HI, Chang WS, Aslam Z, Chung YR. |
| タイトル | Nitrospirillum irinus sp. nov., a diazotrophic bacterium isolated from the rhizosphere soil of Iris and emended description of the genus Nitrospirillum. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
A polyphasic approach was used to characterize a novel nitrogen-fixing bacterial strain, designated YC6995(T), isolated from the rhizosphere soil of Iris ensata var. spontanea (Makino) Nakai inhabiting a wetland located at an altitude of 960 m on Jiri Mountain, Korea. Strain YC6995(T) cells were Gram-negative, and rod-shaped, with motility provided by a single polar flagellum. Optimal growth conditions were 30 °C and pH 7.0. The major fatty acids of strain YC6995(T) were C18:1 ω7c, C18:1 2-OH and C16:0 3-OH. The major respiratory quinone was ubiquinone-10 (Q-10). The polar lipids were phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, phosphatidylglycerol and unidentified glycolipids. The genomic DNA G+C content was 64.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed strain YC6995(T) to form a phyletic lineage with Nitrospirillum amazonense DSM 2787(T) with a high sequence similarity (97.2 %), but it displayed low sequence similarity with other remotely related genera, including Azospirillum (<93 %), Rhodocista (93.1-93.4 %), and Skermanella (91.2-93.3 %) in the family Alphaproteobacteria. Based on the phenotypic, chemotaxonomic, and phylogenetic evidences, strain YC6995(T) represents a novel species within the genus Nitrospirillum, for which the name Nitrospirillum irinus sp. nov. is proposed. The type strain is YC6995(T) (= KACC 13777(T) = DSM 22198(T)). An emended description of the genus Nitrospirillum is also proposed. |
| 巻・号 | 108(3) |
| ページ | 721-9 |
| 公開日 | 2015-9-1 |
| DOI | 10.1007/s10482-015-0528-x |
| PMID | 26155773 |
| MeSH | Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Flagella / physiology Glycolipids / analysis Hydrogen-Ion Concentration Iris / microbiology Locomotion Molecular Sequence Data Nitrogen Fixation Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Republic of Korea Rhizosphere Rhodospirillaceae / classification* Rhodospirillaceae / genetics Rhodospirillaceae / isolation & purification* Rhodospirillaceae / physiology Sequence Analysis, DNA Soil Microbiology* Temperature |
| IF | 2.674 |
| 引用数 | 3 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 21060 |