論文 - 詳細
| RRC ID | 41122 |
|---|---|
| 著者 | Radhika V, Ueda N, Tsuboi Y, Kojima M, Kikuchi J, Kudo T, Sakakibara H. |
| タイトル | Methylated Cytokinins from the Phytopathogen Rhodococcus fascians Mimic Plant Hormone Activity. |
| ジャーナル | Plant Physiol |
| Abstract |
Cytokinins (CKs), a class of phytohormones that regulate plant growth and development, are also synthesized by some phytopathogens to disrupt the hormonal balance and to facilitate niche establishment in their hosts. Rhodococcus fascians harbors the fasciation (fas) locus, an operon encoding several genes homologous to CK biosynthesis and metabolism. This pathogen causes unique leafy gall symptoms reminiscent of CK overproduction; however, bacterial CKs have not been clearly correlated with the severe symptoms, and no virulence-associated unique CKs or analogs have been identified. Here, we report the identification of monomethylated N(6)-(∆(2)-isopentenyl)adenine and dimethylated N(6)-(∆(2)-isopentenyl)adenine (collectively, methylated cytokinins [MeCKs]) from R. fascians. MeCKs were recognized by a CK receptor and up-regulated type-A ARABIDOPSIS THALIANA RESPONSE REGULATOR genes. Treatment with MeCKs inhibited root growth, a hallmark of CK action, whereas the receptor mutant was insensitive. MeCKs were retained longer in planta than canonical CKs and were poor substrates for a CK oxidase/dehydrogenase, suggesting enhanced biological stability. MeCKs were synthesized by S-adenosyl methionine-dependent methyltransferases (MT1 and MT2) that are present upstream of the fas genes. The best substrate for methylation was isopentenyl diphosphate. MT1 and MT2 catalyzed distinct methylation reactions; only the MT2 product was used by FAS4 to synthesize monomethylated N(6)-(∆(2)-isopentenyl)adenine. The MT1 product was dimethylated by MT2 and used as a substrate by FAS4 to produce dimethylated N(6)-(∆(2)-isopentenyl)adenine. Chemically synthesized MeCKs were comparable in activity. Our results strongly suggest that MeCKs function as CK mimics and play a role in this plant-pathogen interaction. |
| 巻・号 | 169(2) |
| ページ | 1118-26 |
| 公開日 | 2015-10-1 |
| DOI | 10.1104/pp.15.00787 |
| PII | pp.15.00787 |
| PMID | 26251309 |
| PMC | PMC4587462 |
| MeSH | Arabidopsis / drug effects Arabidopsis / microbiology* Cytokinins / chemistry* Cytokinins / metabolism* Cytokinins / pharmacology Host-Pathogen Interactions* Isopentenyladenosine / chemistry Isopentenyladenosine / metabolism Methylation Molecular Mimicry Molecular Structure Plant Roots / drug effects Plant Roots / growth & development Rhodococcus / metabolism Rhodococcus / pathogenicity* |
| IF | 6.902 |
| 引用数 | 30 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 6162 |