論文 - 詳細
| RRC ID | 41123 |
|---|---|
| 著者 | Yuliana T, Ebihara K, Suzuki M, Shimonaka C, Amachi S. |
| タイトル | A novel enzyme-based antimicrobial system comprising iodide and a multicopper oxidase isolated from Alphaproteobacterium strain Q-1. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
Alphaproteobacterium strain Q-1 produces an extracellular multicopper oxidase (IOX), which catalyzes iodide (I-) oxidation to form molecular iodine (I2). In this study, the antimicrobial activity of the IOX/iodide system was determined. Both Gram-positive and Gram-negative bacteria tested were killed completely within 5 min by 50 mU mL(-1) of IOX and 10 mM iodide. The sporicidal activity of the system was also tested and compared with a common iodophor, povidone-iodine (PVP-I). IOX (300 mU mL(-1)) killed Bacillus cereus, B. subtilis, and Geobacillus stearothermophilus spores with decimal reduction times of 2.58, 7.62, and 40.9 min, respectively. However, 0.1% PVP-I killed these spores with much longer decimal reduction times of 5.46, 38.0, and 260 min, respectively. To evaluate the more superior sporicidal activity of the IOX system over PVP-I, the amount of free iodine (non-complexed I2) was determined by an equilibrium dialysis technique. The IOX system included more than 40 mg L(-1) of free iodine, while PVP-I included at most 25 mg L(-1) free iodine. Our results suggest that the new enzyme-based antimicrobial system is effective against a wide variety of microorganisms and bacterial spores, and that its strong biocidal activity is due to its high free iodine content, which is probably maintained by re-oxidation of iodide released after oxidation of cell components by I2. |
| 巻・号 | 99(23) |
| ページ | 10011-8 |
| 公開日 | 2015-12-1 |
| DOI | 10.1007/s00253-015-6862-0 |
| PII | 10.1007/s00253-015-6862-0 |
| PMID | 26254787 |
| MeSH | Alphaproteobacteria / enzymology Alphaproteobacteria / metabolism* Anti-Bacterial Agents / isolation & purification Anti-Bacterial Agents / metabolism Anti-Bacterial Agents / pharmacology* Gram-Negative Bacteria / drug effects Gram-Negative Bacteria / physiology Gram-Positive Bacteria / drug effects Gram-Positive Bacteria / physiology Iodides / pharmacology* Microbial Viability / drug effects Oxidoreductases / isolation & purification Oxidoreductases / metabolism Oxidoreductases / pharmacology* Spores, Bacterial / drug effects Time Factors |
| IF | 3.53 |
| 引用数 | 3 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM17846 JCM 2152 JCM 1651 JCM 1239 JCM 20624 JCM 7577 JCM 2360 JCM 9070 JCM 7255 |