Reference - Detail
| RRC ID | 41187 |
|---|---|
| Author | Sugahara H, Odamaki T, Fukuda S, Kato T, Xiao JZ, Abe F, Kikuchi J, Ohno H. |
| Title | Probiotic Bifidobacterium longum alters gut luminal metabolism through modification of the gut microbial community. |
| Journal | Sci Rep |
| Abstract |
Probiotics are well known as health-promoting agents that modulate intestinal microbiota. However, the molecular mechanisms underlying this effect remain unclear. Using gnotobiotic mice harboring 15 strains of predominant human gut-derived microbiota (HGM), we investigated the effects of Bifidobacterium longum BB536 (BB536-HGM) supplementation on the gut luminal metabolism. Nuclear magnetic resonance (NMR)-based metabolomics showed significantly increased fecal levels of pimelate, a precursor of biotin, and butyrate in the BB536-HGM group. In addition, the bioassay revealed significantly elevated fecal levels of biotin in the BB536-HGM group. Metatranscriptomic analysis of fecal microbiota followed by an in vitro bioassay indicated that the elevated biotin level was due to an alteration in metabolism related to biotin synthesis by Bacteroides caccae in this mouse model. Furthermore, the proportion of Eubacterium rectale, a butyrate producer, was significantly higher in the BB536-HGM group than in the group without B. longum BB536 supplementation. Our findings help to elucidate the molecular basis underlying the effect of B. longum BB536 on the gut luminal metabolism through its interactions with the microbial community. |
| Volume | 5 |
| Pages | 13548 |
| Published | 2015-8-28 |
| DOI | 10.1038/srep13548 |
| PII | srep13548 |
| PMID | 26315217 |
| PMC | PMC4552000 |
| MeSH | Animals Bifidobacterium / drug effects Bifidobacterium / genetics Bifidobacterium / metabolism* Biotin / metabolism Feces / microbiology Gastrointestinal Tract / drug effects Gastrointestinal Tract / microbiology* Gene Expression Regulation / drug effects Germ-Free Life Humans Magnetic Resonance Spectroscopy Metabolome / drug effects Metabolomics Mice Microbiota / drug effects* Probiotics / pharmacology* RNA, Messenger / genetics RNA, Messenger / metabolism Solubility Species Specificity Water |
| IF | 3.998 |
| Times Cited | 63 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 43 |
| Altmetric score changes over past 6months | 48.2 |
| Resource | |
| General Microbes | JCM 15632 JCM 5828 JCM 9498 JCM 5824 JCM 5827 JCM 5826 JCM 6567 JCM 1432 JCM 10188 JCM 5825 |