RRC ID 41192
Author Garrido D, Ruiz-Moyano S, Lemay DG, Sela DA, German JB, Mills DA.
Title Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria.
Journal Sci Rep
Abstract Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO, and compared the global transcriptomes of representative isolates on major HMO by RNA-seq. While B. infantis displayed homogeneous HMO-utilization patterns, B. bifidum were more diverse and some strains did not use fucosyllactose (FL) or sialyllactose (SL). Transcriptomes of B. bifidum SC555 and B. infantis ATCC 15697 showed that utilization of pooled HMO is similar to neutral HMO, while transcriptomes for growth on FL were more similar to lactose than HMO in B. bifidum. Genes linked to HMO-utilization were upregulated by neutral HMO and SL, but not by FL in both species. In contrast, FL induced the expression of alternative gene clusters in B. infantis. Results also suggest that B. bifidum SC555 does not utilize fucose or sialic acid from HMO. Surprisingly, expression of orthologous genes differed between both bifidobacteria even when grown on identical substrates. This study highlights two major strategies found in Bifidobacterium species to process HMO, and presents detailed information on the close relationship between HMO and infant-gut bifidobacteria.
Volume 5
Pages 13517
Published 2015-9-4
DOI 10.1038/srep13517
PII srep13517
PMID 26337101
PMC PMC4559671
MeSH Animals Bifidobacterium / metabolism* Gastrointestinal Microbiome / physiology Humans Intestines / metabolism* Intestines / microbiology* Milk, Human / chemistry* Milk, Human / microbiology Oligosaccharides / metabolism* Transcription Factors / metabolism* Transcriptome / physiology
IF 4.122
Times Cited 12
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 1254 JCM 1255 JCM 1209 JCM 7002 JCM 7003 JCM 7004 JCM 10602 JCM 7007 JCM 7009 JCM 7011