論文 - 詳細
| RRC ID | 41192 |
|---|---|
| 著者 | Garrido D, Ruiz-Moyano S, Lemay DG, Sela DA, German JB, Mills DA. |
| タイトル | Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria. |
| ジャーナル | Sci Rep |
| Abstract |
Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO, and compared the global transcriptomes of representative isolates on major HMO by RNA-seq. While B. infantis displayed homogeneous HMO-utilization patterns, B. bifidum were more diverse and some strains did not use fucosyllactose (FL) or sialyllactose (SL). Transcriptomes of B. bifidum SC555 and B. infantis ATCC 15697 showed that utilization of pooled HMO is similar to neutral HMO, while transcriptomes for growth on FL were more similar to lactose than HMO in B. bifidum. Genes linked to HMO-utilization were upregulated by neutral HMO and SL, but not by FL in both species. In contrast, FL induced the expression of alternative gene clusters in B. infantis. Results also suggest that B. bifidum SC555 does not utilize fucose or sialic acid from HMO. Surprisingly, expression of orthologous genes differed between both bifidobacteria even when grown on identical substrates. This study highlights two major strategies found in Bifidobacterium species to process HMO, and presents detailed information on the close relationship between HMO and infant-gut bifidobacteria. |
| 巻・号 | 5 |
| ページ | 13517 |
| 公開日 | 2015-9-4 |
| DOI | 10.1038/srep13517 |
| PII | srep13517 |
| PMID | 26337101 |
| PMC | PMC4559671 |
| MeSH | Animals Bifidobacterium / metabolism* Gastrointestinal Microbiome / physiology Humans Intestinal Mucosa / metabolism* Intestines / microbiology* Milk, Human / chemistry* Milk, Human / microbiology Oligosaccharides / metabolism* Transcription Factors / metabolism* Transcriptome / physiology |
| IF | 3.998 |
| 引用数 | 67 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM 1254 JCM 1255 JCM 1209 JCM 7002 JCM 7003 JCM 7004 JCM 10602 JCM 7007 JCM 7009 JCM 7011 |