| RRC ID |
41192
|
| 著者 |
Garrido D, Ruiz-Moyano S, Lemay DG, Sela DA, German JB, Mills DA.
|
| タイトル |
Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO, and compared the global transcriptomes of representative isolates on major HMO by RNA-seq. While B. infantis displayed homogeneous HMO-utilization patterns, B. bifidum were more diverse and some strains did not use fucosyllactose (FL) or sialyllactose (SL). Transcriptomes of B. bifidum SC555 and B. infantis ATCC 15697 showed that utilization of pooled HMO is similar to neutral HMO, while transcriptomes for growth on FL were more similar to lactose than HMO in B. bifidum. Genes linked to HMO-utilization were upregulated by neutral HMO and SL, but not by FL in both species. In contrast, FL induced the expression of alternative gene clusters in B. infantis. Results also suggest that B. bifidum SC555 does not utilize fucose or sialic acid from HMO. Surprisingly, expression of orthologous genes differed between both bifidobacteria even when grown on identical substrates. This study highlights two major strategies found in Bifidobacterium species to process HMO, and presents detailed information on the close relationship between HMO and infant-gut bifidobacteria.
|
| 巻・号 |
5
|
| ページ |
13517
|
| 公開日 |
2015-9-4
|
| DOI |
10.1038/srep13517
|
| PII |
srep13517
|
| PMID |
26337101
|
| PMC |
PMC4559671
|
| MeSH |
Animals
Bifidobacterium / metabolism*
Gastrointestinal Microbiome / physiology
Humans
Intestinal Mucosa / metabolism*
Intestines / microbiology*
Milk, Human / chemistry*
Milk, Human / microbiology
Oligosaccharides / metabolism*
Transcription Factors / metabolism*
Transcriptome / physiology
|
| IF |
3.998
|
| 引用数 |
67
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 1254
JCM 1255
JCM 1209
JCM 7002
JCM 7003
JCM 7004
JCM 10602
JCM 7007
JCM 7009
JCM 7011 |
