Reference - Detail
| RRC ID | 41229 |
|---|---|
| Author | Nakano M. |
| Title | Development of a Quantitative PCR Assay for Thermophilic Spore-Forming Geobacillus stearothermophilus in Canned Food. |
| Journal | Biocontrol Sci |
| Abstract |
The thermophilic spore forming bacteria Geobacillus stearothermophilus is recognized as a major cause of spoilage in canned food. A quantitative real-time PCR assay was developed to specifically detect and quantify the species G. stearothermophilus in samples from canned food. The selected primer pairs amplified a 163-bp fragment of the 16S rRNA gene in a specific PCR assay with a detection limit of 12.5 fg of pure culture DNA, corresponding to DNA extracted from approximately 0.7 CFU/mL of G. stearothermophilus. Analysis showed that the bacterial species G. stearothermophilus was not detected in any canned food sample. Our approach presented here will be useful for tracking or quantifying species G. stearotethermophilus in canned food and ingredients. |
| Volume | 20(3) |
| Pages | 221-7 |
| Published | 2015-1-1 |
| DOI | 10.4265/bio.20.221 |
| PMID | 26412704 |
| MeSH | DNA, Bacterial / genetics DNA, Ribosomal / genetics Food Microbiology / methods Food, Preserved / microbiology* Geobacillus stearothermophilus / genetics Geobacillus stearothermophilus / isolation & purification* RNA, Ribosomal, 16S / genetics Real-Time Polymerase Chain Reaction / methods* Spores / genetics Spores / isolation & purification* |
| IF | 0.833 |
| Times Cited | 2 |
| WOS Category | BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| General Microbes | JCM 9319 JCM 9320 |