| RRC ID |
41261
|
| Author |
Hirakata Y, Oshiki M, Kuroda K, Hatamoto M, Kubota K, Yamaguchi T, Harada H, Araki N.
|
| Title |
Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge.
|
| Journal |
Microbes Environ
|
| Abstract |
The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA gene sequences from the prokaryotes Methanoregula boonei and Clostridium aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these prokaryotes were localized within Metopus cells. These results identify M. boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus ciliates.
|
| Volume |
30(4)
|
| Pages |
335-8
|
| Published |
2015-1-1
|
| DOI |
10.1264/jsme2.ME15154
|
| PMID |
26639580
|
| PMC |
PMC4676557
|
| MeSH |
Biota*
Ciliophora / microbiology*
Clostridium / classification
Clostridium / genetics
Clostridium / isolation & purification*
Cluster Analysis
DNA, Archaeal / chemistry
DNA, Archaeal / genetics
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
In Situ Hybridization, Fluorescence
Methanomicrobiales / classification
Methanomicrobiales / genetics
Methanomicrobiales / isolation & purification*
Microbial Consortia
Microscopy, Electron, Transmission
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sewage / microbiology*
Symbiosis*
|
| IF |
2.575
|
| Times Cited |
14
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 3805 |
