論文 - 詳細
| RRC ID | 41324 |
|---|---|
| 著者 | Soeda S, Ishida S, Honda O, Shimeno H, Nagamatsu A. |
| タイトル | Aminated fucoidan promotes the invasion of 3 LL cells through reconstituted basement membrane: its possible mechanism of action. |
| ジャーナル | Cancer Lett |
| Abstract |
Fucoidan is reported to have an antimetastatic activity. In the present study, we prepared an amino group-introduced derivative of fucoidan and examined its effect on the invasion of 3 LL cells through a reconstituted basement membrane (MatrigelTM). Unlike native fucoidan, the aminated derivative promoted the tumor cell invasion: maximal promotion (240% of control invasion) was obtained with 5 micrograms/ml. However, with higher concentrations (10-30 micrograms/ml) of the fucoidan derivative, the promotion was gradually reduced to 130% of control. Both native and aminated fucoidans inhibited specifically the attachment of 3 LL cells to laminin. Interestingly, aminated fucoidan, unlike the native one, promoted the tumor cell adhesion to immobilized synthetic laminin B 1 chain peptide, YIGSR, over a concentration range of 0.5-5 micrograms/ml. Higher concentrations (7-20 micrograms/ml) of the aminated derivative suppressed the adhesive ability of 3 LL cells to YIGSR. 3 LL cells secreted a 50-kDa form of urokinase-type plasminogen activator (u-PA) in the culture medium. Addition of aminated fucoidan (5 micrograms/ml) or YIGSR (10 micrograms/ml) resulted in a 1.7-fold increase in u-PA activity. This effect was enhanced up to 3.5-fold when both substances were simultaneously added. The addition of native fucoidan had no effect. The present results suggest that the 67-kDa receptor-mediated binding of 3 LL cells to laminin activates their invasiveness, especially by enhancing the extracellular u-PA levels. Aminated, but not native, fucoidan may act to enhance the laminin-receptor interaction at the limited concentration range. |
| 巻・号 | 85(1) |
| ページ | 133-8 |
| 公開日 | 1994-9-30 |
| DOI | 10.1016/0304-3835(94)90249-6 |
| PII | 0304-3835(94)90249-6 |
| PMID | 7923097 |
| MeSH | Amination Amino Acid Sequence Animals Anticoagulants / toxicity* Basement Membrane / drug effects Basement Membrane / physiology Carcinoma, Lewis Lung / metabolism Carcinoma, Lewis Lung / pathology* Carcinoma, Lewis Lung / ultrastructure* Collagen / metabolism Fibronectins / metabolism Laminin / metabolism Molecular Sequence Data Neoplasm Invasiveness Polysaccharides / metabolism Polysaccharides / toxicity* Receptors, Laminin / physiology Tumor Cells, Cultured / drug effects Urokinase-Type Plasminogen Activator / metabolism |
| IF | 7.36 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LLC(RCB0558) |