| RRC ID |
41329
|
| 著者 |
Kim YH, Iida T, Fujita T, Terada S, Kitayama A, Ueda H, Prochownik EV, Suzuki E.
|
| タイトル |
Establishment of an apoptosis-resistant and growth-controllable cell line by transfecting with inducible antisense c-Jun gene.
|
| ジャーナル |
Biotechnol Bioeng
|
| Abstract |
F-MEL cells were transfected with the c-jun antisense gene located downstream of a glucocorticoid-inducible MMTV promoter, and the obtained cells were named c-jun AS cells. When the c-jun AS cells were treated with dexamethasone (DEX) in DMEM supplemented with 10% serum, the growth of the cells was completely suppressed for a duration of 16 days with a high cell viability exceeding 86%. The c-jun expression in the c-jun AS cells was suppressed moderately in the absence of DEX and strongly in the presence of DEX. The c-jun AS cells grew well and reached a density of 10(6) cells/mL without supplementation of any serum components. Viability was greater than 80% after the cells had been cultured for 8 days in the absence of DEX. The c-jun AS cells stayed at a constant cell density and high viability above 80% for 8 days when they were cultured in the presence of DEX under serum deprivation. In contrast, the wild type F-MEL cells were unable to grow and died by apoptosis in 3 days under serum deprivation. Internucleosomal cleavage of DNA, a landmark of apoptosis, was clearly detectable. Thus the c-jun AS cell line that is resistant to apoptosis induced by serum deprivation and can reversibly and viably be growth-arrested was established. A dual-signal model was proposed to explain the experimental result, the interlinked regulation of apoptosis, and growth by c-jun.
|
| 巻・号 |
58(1)
|
| ページ |
65-72
|
| 公開日 |
1998-4-5
|
| DOI |
10.1002/(sici)1097-0290(19980405)58:1<65::aid-bit7>3.0.co;2-s
|
| PII |
10.1002/(SICI)1097-0290(19980405)58:1<65::AID-BIT7>3.0.CO;2-S
|
| PMID |
10099262
|
| MeSH |
Animals
Apoptosis / genetics*
Cell Division / drug effects
Dexamethasone / pharmacology
Mice
Oligonucleotides, Antisense / genetics*
Proto-Oncogene Proteins c-jun / genetics*
Transfection*
Tumor Cells, Cultured
|
| IF |
4.002
|
| 引用数 |
53
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
