論文 - 詳細
| RRC ID | 41329 |
|---|---|
| 著者 | Kim YH, Iida T, Fujita T, Terada S, Kitayama A, Ueda H, Prochownik EV, Suzuki E. |
| タイトル | Establishment of an apoptosis-resistant and growth-controllable cell line by transfecting with inducible antisense c-Jun gene. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
F-MEL cells were transfected with the c-jun antisense gene located downstream of a glucocorticoid-inducible MMTV promoter, and the obtained cells were named c-jun AS cells. When the c-jun AS cells were treated with dexamethasone (DEX) in DMEM supplemented with 10% serum, the growth of the cells was completely suppressed for a duration of 16 days with a high cell viability exceeding 86%. The c-jun expression in the c-jun AS cells was suppressed moderately in the absence of DEX and strongly in the presence of DEX. The c-jun AS cells grew well and reached a density of 10(6) cells/mL without supplementation of any serum components. Viability was greater than 80% after the cells had been cultured for 8 days in the absence of DEX. The c-jun AS cells stayed at a constant cell density and high viability above 80% for 8 days when they were cultured in the presence of DEX under serum deprivation. In contrast, the wild type F-MEL cells were unable to grow and died by apoptosis in 3 days under serum deprivation. Internucleosomal cleavage of DNA, a landmark of apoptosis, was clearly detectable. Thus the c-jun AS cell line that is resistant to apoptosis induced by serum deprivation and can reversibly and viably be growth-arrested was established. A dual-signal model was proposed to explain the experimental result, the interlinked regulation of apoptosis, and growth by c-jun. |
| 巻・号 | 58(1) |
| ページ | 65-72 |
| 公開日 | 1998-4-5 |
| DOI | 10.1002/(sici)1097-0290(19980405)58:1<65::aid-bit7>3.0.co;2-s |
| PII | 10.1002/(SICI)1097-0290(19980405)58:1<65::AID-BIT7>3.0.CO;2-S |
| PMID | 10099262 |
| MeSH | Animals Apoptosis / genetics* Cell Division / drug effects Dexamethasone / pharmacology Mice Oligonucleotides, Antisense / genetics* Proto-Oncogene Proteins c-jun / genetics* Transfection* Tumor Cells, Cultured |
| IF | 4.002 |
| 引用数 | 53 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Friend murine erythroleukemia |