| RRC ID |
41335
|
| 著者 |
Suzuki J, Ohnsihi H, Shibata H, Wada A, Hirayama T, Iiri T, Ueda N, Kanamaru C, Tsuchida T, Mashima H, Yasuda H, Fujita T.
|
| タイトル |
Dynamin is involved in human epithelial cell vacuolation caused by the Helicobacter pylori-produced cytotoxin VacA.
|
| ジャーナル |
J Clin Invest
|
| Abstract |
The Helicobacter pylori-produced cytotoxin VacA induces intracellular vacuolation. To elucidate the molecular mechanism of vacuole formation by VacA, we examined the participation of dynamin, a GTPase functioning in intracellular vesicle formation, in human HeLa cells. Immunocytochemistry revealed that endogenous dynamin was localized to vacuoles induced by VacA. In cells transiently transfected with a GTPase-defective (dominant-negative) dynamin mutant, VacA failed to induce vacuolation. In contrast, VacA did induce vacuolation in cells transiently transfected with wild-type dynamin. Furthermore, under VacA treatment, neutral red dye uptake, a parameter of VacA-induced vacuolation, was inhibited in cells stably transfected with the dominant-negative dynamin mutant. In contrast, uptake was markedly enhanced in cells stably transfected with wild-type dynamin. Moreover, VacA cytopathic effects on the viability of HeLa cells were inhibited in cells stably transfected with dominant-negative dynamin-1. Sequential immunocytochemical observation confirmed that expression of dominant-negative dynamin did not affect VacA attachment to or internalization into HeLa cells. We suggest that dynamin is involved in the intracellular vacuolation induced by VacA.
|
| 巻・号 |
107(3)
|
| ページ |
363-70
|
| 公開日 |
2001-2-1
|
| DOI |
10.1172/JCI10254
|
| PMID |
11160160
|
| PMC |
PMC199191
|
| MeSH |
Bacterial Proteins / antagonists & inhibitors
Bacterial Proteins / biosynthesis*
Cell Survival
Cytotoxins / biosynthesis*
Duodenal Diseases / microbiology
Dynamin I
Dynamins
Epithelial Cells / metabolism*
Fluorescent Antibody Technique
GTP Phosphohydrolases / genetics
GTP Phosphohydrolases / metabolism*
HeLa Cells
Helicobacter pylori / metabolism*
Humans
Plasmids
Transfection
|
| IF |
11.864
|
| 引用数 |
37
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
