論文 - 詳細
| RRC ID | 41357 |
|---|---|
| 著者 | Nagasawa M, Kanzaki M, Iino Y, Morishita Y, Kojima I. |
| タイトル | Identification of a novel chloride channel expressed in the endoplasmic reticulum, golgi apparatus, and nucleus. |
| ジャーナル | J Biol Chem |
| Abstract |
MID-1 is a Saccharomyces cerevisiae gene encoding a stretch-activated channel. Using MID-1 as a molecular probe, we isolated rat cDNA encoding a protein with four putative transmembrane domains. This gene encoded a protein of 541 amino acids. We also cloned the human homologue, which encoded 551 amino acids. Messenger RNA for this gene was expressed abundantly in the testis and moderately in the spleen, liver, kidney, heart, brain, and lung. In the testis, immunoreactivity of the gene product was detected both in the cytoplasm and the nucleus. When expressed in Chinese hamster ovary cells, the gene product was located in intracellular compartments including endoplasmic reticulum and the Golgi apparatus. When microsome fraction obtained from the transfected cells, but not from mock-transfected cells, was incorporated into the lipid bilayer, an anion channel activity was detected. Unitary conductance was 70 picosiemens in symmetric 150 mm KCl solution. We designated this gene Mid-1-related chloride channel (MCLC). MCLC encodes a new class of chloride channel expressed in intracellular compartments. |
| 巻・号 | 276(23) |
| ページ | 20413-8 |
| 公開日 | 2001-6-8 |
| DOI | 10.1074/jbc.M100366200 |
| PII | S0021-9258(19)40490-0 |
| PMID | 11279057 |
| MeSH | Amino Acid Sequence Animals Base Sequence CHO Cells Cell Nucleus / metabolism* Chloride Channels / chemistry* Chloride Channels / genetics Chloride Channels / metabolism Cloning, Molecular Cricetinae DNA, Complementary Endoplasmic Reticulum / metabolism* Golgi Apparatus / metabolism* Molecular Sequence Data Rats Sequence Homology, Amino Acid Xenopus |
| IF | 4.238 |
| 引用数 | 27 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |