Reference - Detail
| RRC ID | 41360 |
|---|---|
| Author | Ito H, Akiyama H, Iguchi H, Iyama K, Miyamoto M, Ohsawa K, Nakamura T. |
| Title | Molecular cloning and biological activity of a novel lysyl oxidase-related gene expressed in cartilage. |
| Journal | J Biol Chem |
| Abstract |
We cloned a cDNA encoding a novel lysyl oxidase-related protein, named LOXC, by suppression subtractive hybridization between differentiated and calcified ATDC5 cells, a clonal mouse chondrogenic EC cell line. The deduced amino acid sequence of mouse LOXC consists of 757 amino acids and shows 50% identity with that of mouse lysyl oxidase. Northern blot analysis showed a distinct hybridization band of 5.4 kilobases, and Western blot analysis showed an immunoreactive band at 82 kilodaltons. Expression of LOXC mRNA was detected in osteoblastic MC3T3-E1 cells and embryonic fibroblast C3H10T1/2 cells, whereas none of NIH3T3 fibroblasts and myoblastic C2C12 cells expressed LOXC mRNA in vitro. Moreover, the LOXC mRNA and protein levels dramatically increased throughout a process of chondrogenic differentiation in ATDC5 cells. In vivo, LOXC gene expression was localized in hypertrophic and calcified chondrocytes of growth plates in adult mice. The conditioned media of COS-7 cells transfected with the full-length LOXC cDNA showed the lysyl oxidase activity in both type I and type II collagens derived from chick embryos, and these activities of LOXC were inhibited by beta-aminopropionitrile, a specific inhibitor of lysyl oxidase. Our data indicate that LOXC is expressed in cartilage in vivo and modulates the formation of a collagenous extracellular matrix. |
| Volume | 276(26) |
| Pages | 24023-9 |
| Published | 2001-6-29 |
| DOI | 10.1074/jbc.M100861200 |
| PII | S0021-9258(20)78408-5 |
| PMID | 11292829 |
| MeSH | Amino Acid Oxidoreductases* Amino Acid Sequence Animals Animals, Newborn Base Sequence Blotting, Western COS Cells Cartilage / enzymology* Cell Line Cloning, Molecular Growth Plate / enzymology Mice Molecular Sequence Data Protein-Lysine 6-Oxidase / genetics* Protein-Lysine 6-Oxidase / metabolism* RNA, Messenger / biosynthesis Sequence Homology, Amino Acid Tissue Distribution Transcription, Genetic Transfection |
| IF | 4.238 |
| Times Cited | 51 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 24 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | NIH3T3-3(RCB0150) C2C12(RCB0987) 10T1/2(RCB0247) MC3T3-E1(RCB1126) |