RRC ID |
41372
|
著者 |
Lee D, Katayama H, Akao T, Maeda M, Tanaka R, Yamashita S, Saitoh H, Mizuki E, Ohba M.
|
タイトル |
A 28 kDa protein of the Bacillus thuringiensis serovar shandongiensis isolate 89-T-34-22 induces a human leukemic cell-specific cytotoxicity.
|
ジャーナル |
Biochim Biophys Acta
|
Abstract |
A 28 kDa protein that exhibits cytocidal activity specific for human leukemic T (MOLT-4) cells was purified from proteinase K-digested parasporal inclusion of a Bacillus thuringiensis serovar shandongiensis isolate. The N-terminal sequence of the protein was identical with that of the 32 kDa protein, regarded as a protoxin, of the inclusion proteins. The median effective concentration of this protein was 0.23 microg/ml against MOLT-4 cells and its specific activity was 7.9 times greater than that of the whole inclusion proteins. The 28 kDa protein induced necrosis-like cytotoxicity against MOLT-4 cells and the cytopathic effect with the passage of time was characterized by cell swelling, nuclear membrane isolation and chromatin condensation.
|
巻・号 |
1547(1)
|
ページ |
57-63
|
公開日 |
2001-5-5
|
DOI |
10.1016/s0167-4838(01)00169-8
|
PII |
S0167483801001698
|
PMID |
11343791
|
MeSH |
Antineoplastic Agents / pharmacology
Bacillus thuringiensis / chemistry
Bacillus thuringiensis / isolation & purification*
Bacterial Proteins / isolation & purification*
Bacterial Proteins / pharmacology
Cell Nucleus / drug effects
Chemical Fractionation
Chromatography, Ion Exchange
Crotalid Venoms
Electrophoresis, Polyacrylamide Gel
Endopeptidase K
Endotoxins / isolation & purification
HeLa Cells / drug effects
Humans
Leukemia, T-Cell
Mitochondrial Swelling / drug effects
Naphthols
Neurotoxins
Nuclear Envelope / drug effects
Tumor Cells, Cultured / drug effects
|
IF |
3.411
|
引用数 |
22
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
MOLT-4(RCB0206)
HeLa(RCB0007) |