RRC ID 41372
Author Lee D, Katayama H, Akao T, Maeda M, Tanaka R, Yamashita S, Saitoh H, Mizuki E, Ohba M.
Title A 28 kDa protein of the Bacillus thuringiensis serovar shandongiensis isolate 89-T-34-22 induces a human leukemic cell-specific cytotoxicity.
Journal Biochim. Biophys. Acta
Abstract A 28 kDa protein that exhibits cytocidal activity specific for human leukemic T (MOLT-4) cells was purified from proteinase K-digested parasporal inclusion of a Bacillus thuringiensis serovar shandongiensis isolate. The N-terminal sequence of the protein was identical with that of the 32 kDa protein, regarded as a protoxin, of the inclusion proteins. The median effective concentration of this protein was 0.23 microg/ml against MOLT-4 cells and its specific activity was 7.9 times greater than that of the whole inclusion proteins. The 28 kDa protein induced necrosis-like cytotoxicity against MOLT-4 cells and the cytopathic effect with the passage of time was characterized by cell swelling, nuclear membrane isolation and chromatin condensation.
Volume 1547(1)
Pages 57-63
Published 2001-5-5
DOI 10.1016/s0167-4838(01)00169-8
PII S0167483801001698
PMID 11343791
MeSH Antineoplastic Agents / pharmacology Bacillus thuringiensis / chemistry Bacillus thuringiensis / isolation & purification* Bacterial Proteins / isolation & purification* Bacterial Proteins / pharmacology Cell Nucleus / drug effects Chemical Fractionation Chromatography, Ion Exchange Crotalid Venoms Electrophoresis, Polyacrylamide Gel Endopeptidase K Endotoxins / isolation & purification HeLa Cells / drug effects Humans Leukemia, T-Cell Mitochondrial Swelling / drug effects Naphthols Neurotoxins Nuclear Envelope / drug effects Tumor Cells, Cultured / drug effects
IF 3.438
Times Cited 21
Human and Animal Cells