RRC ID 41375
著者 Saika M, Inoue D, Kido S, Matsumoto T.
タイトル 17beta-estradiol stimulates expression of osteoprotegerin by a mouse stromal cell line, ST-2, via estrogen receptor-alpha.
ジャーナル Endocrinology
Abstract Osteoprotegerin (OPG) is a recently identified member of the tumor necrosis factor (TNF) receptor superfamily that regulates bone mass through an inhibitory action on osteoclast differentiation and function. To determine its potential roles of OPG in pathological changes in bone metabolism caused by estrogen deficiency, we investigated effects of estrogen on OPG expression by a mouse stromal cell line, ST-2, in vitro. Treatment of ST-2 cells with 17beta-E(2) resulted in up-regulation of OPG expression at both the messenger RNA and protein levels. The effect was time and dose dependent and steroid specific. The stimulatory action of 17beta-E(2) on OPG expression appeared to be mediated by the estrogen receptor-alpha (ERalpha) subtype because stable overexpression of ERalpha, but not of ERbeta, enhanced the OPG induction by 17beta-E(2). Moreover, estrogen withdrawal after 5-day pretreatment, mimicking the event occurring in vivo at menopause, dramatically diminished the expression of OPG. These findings suggest that down-regulation of OPG after estrogen withdrawal contributes to the enhanced osteoclastic bone resorption and bone loss after menopause by enhancing RANK ligand-RANK system that lies downstream of a large number of bone-resorbing cytokines.
巻・号 142(6)
ページ 2205-12
公開日 2001-6-1
DOI 10.1210/endo.142.6.8220
PMID 11356664
MeSH Animals Cell Line Dose-Response Relationship, Drug Estradiol / administration & dosage Estradiol / pharmacology* Estrogen Receptor alpha Gene Expression / drug effects* Glycoproteins / analysis Glycoproteins / genetics* Humans Kinetics Mice Osteoprotegerin RNA, Messenger / analysis Receptors, Cytoplasmic and Nuclear / analysis Receptors, Cytoplasmic and Nuclear / genetics* Receptors, Estrogen / drug effects Receptors, Estrogen / physiology* Receptors, Tumor Necrosis Factor Stromal Cells / metabolism*
IF 3.934
引用数 148
リソース情報
ヒト・動物細胞 ST2(RCB0224)