| RRC ID |
41380
|
| 著者 |
Pohle T, Becker JC, Markmann A, Lügering N, Pauels HG, Konturek JW, Domschke W.
|
| タイトル |
Aspirin effects on gastric epithelial cell proliferation and cytokine expression.
|
| ジャーナル |
Microsc Res Tech
|
| Abstract |
Aspirin is known to cause gastric injury and to delay ulcer healing. The effects of aspirin on gastric epithelial cell function are heterogeneous; in contrast to injuring the mucosa, aspirin may also act beneficially by inducing adaptation; a mechanism that is poorly understood. We aimed to document the effects of different doses of aspirin on gastric epithelial cell function defined as proliferation, and secretion as well as mRNA expression of cytokines. Furthermore, we studied the effects of aspirin pretreatment on cytokine secretion as a potential element of gastric adaptation. The proliferative activity of three different gastric epithelial cell lines (AGS, KATO III, RGM-1) was assessed by (3)H-thymidine incorporation; secretion of growth factors PDGF-AB and VEGF into culture supernatant was documented by ELISA. mRNA transcripts of both cytokines were quantified by real time RT-PCR. Low doses of aspirin did not alter the proliferative dynamics in two of the three studied cell lines; high doses abolished proliferation. Secretion of PDGF-AB and VEGF increased during the first days of low dose aspirin exposition; higher concentrations led to a depletion of cytokines after an initial liberation in the case of VEGF, mRNA of which was also dose-dependently increased by aspirin. Seven-day pretreatment with low amounts of aspirin did not alter the secretory response of the epithelia caused by higher doses of this drug. The secretion of cytokines and proliferation of gastric epithelial cells are adversely effected by aspirin in a similarly dose-dependent fashion as the intended effects of this drug on platelet function and pain relief.
|
| 巻・号 |
53(5)
|
| ページ |
354-9
|
| 公開日 |
2001-6-1
|
| DOI |
10.1002/jemt.1103
|
| PII |
10.1002/jemt.1103
|
| PMID |
11376496
|
| MeSH |
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Anti-Inflammatory Agents, Non-Steroidal / toxicity
Aspirin / pharmacology*
Aspirin / toxicity
Cell Division / drug effects
Cell Line
Cytokines / biosynthesis
Cytokines / metabolism*
Dose-Response Relationship, Drug
Endothelial Growth Factors / biosynthesis
Endothelial Growth Factors / metabolism
Enzyme-Linked Immunosorbent Assay
Gastric Mucosa / cytology
Gastric Mucosa / drug effects*
Gastric Mucosa / metabolism
Humans
Lymphokines / biosynthesis
Lymphokines / metabolism
Platelet-Derived Growth Factor / biosynthesis
Platelet-Derived Growth Factor / metabolism
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
|
| IF |
2.117
|
| 引用数 |
7
|
|
WOS 分野
|
MICROSCOPY
BIOLOGY
ANATOMY & MORPHOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
