論文 - 詳細
| RRC ID | 41381 |
|---|---|
| 著者 | Hirano R, Igarashi O, Kondo K, Itakura H, Matsumoto A. |
| タイトル | Regulation by long-chain fatty acids of the expression of cholesteryl ester transfer protein in HepG2 cells. |
| ジャーナル | Lipids |
| Abstract |
Cholesteryl ester transfer protein (CETP) is an important determinant of lipoprotein function, especially high density lipoprotein (HDL) metabolism, and contributes to the regulation of plasma HDL levels. Since saturated and polyunsaturated fatty acids (FA) appear to influence the CETP activity differently, we decided to investigate the effects of FA on the expression of CETP mRNA in HepG2 cells using an RNA blot hybridization analysis. Long-chain FA (>18 carbons) at a 0.5 mM concentration were added to the medium and incubated with cells for 48 h at 37 degrees C under 5% CO2. After treatment with 0.5 mM arachidonic (AA), eicosapentaenoic (EPA), and docosahexaenoic acid (DHA), the levels of CETP mRNA were less than 50% of the control levels (AA, P = 0.0005; EPA, P < 0.01; DHA, P < 0.0001), with a corresponding significant decrease in the CETP mass. These results suggest that FA regulate the gene expression of CETP in HepG2 and this effect is dependent upon the degree of unsaturation of the acyl carbon chain in FA. |
| 巻・号 | 36(4) |
| ページ | 401-6 |
| 公開日 | 2001-4-1 |
| DOI | 10.1007/s11745-001-0735-3 |
| PMID | 11383693 |
| MeSH | Arachidonic Acid / pharmacology Carcinoma, Hepatocellular / metabolism* Carrier Proteins / genetics* Cholesterol Ester Transfer Proteins Docosahexaenoic Acids / pharmacology Eicosapentaenoic Acid / pharmacology Fatty Acids / metabolism Gene Expression Regulation / drug effects* Glycoproteins* Humans RNA, Messenger / analysis Tumor Cells, Cultured |
| IF | 1.919 |
| 引用数 | 21 |
| WOS 分野 | NUTRITION & DIETETICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 144 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB0459) |