論文 - 詳細
| RRC ID | 4139 |
|---|---|
| 著者 | Kawakami K. |
| タイトル | Tol2: a versatile gene transfer vector in vertebrates. |
| ジャーナル | Genome Biol |
| Abstract |
The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has 200 and 150 base pairs of DNA from the left and right ends of the Tol2 sequence, respectively. These sequences contain essential terminal inverted repeats and subterminal sequences. DNA inserts of fairly large sizes (as large as 11 kilobases) can be cloned between these sequences without reducing transpositional activity. The Tol2 transposon system has been shown to be active in all vertebrate cells tested thus far, including zebrafish, Xenopus, chicken, mouse, and human. In this review I describe and discuss how the Tol2 transposon is being applied to transgenic studies in these vertebrates, and possible future applications. |
| 巻・号 | 8 Suppl 1(Suppl 1) |
| ページ | S7 |
| 公開日 | 2007-1-1 |
| DOI | 10.1186/gb-2007-8-s1-s7 |
| PII | gb-2007-8-s1-s7 |
| PMID | 18047699 |
| PMC | PMC2106836 |
| MeSH | Animals DNA Transposable Elements / genetics* Gene Transfer Techniques* Genetic Vectors* Germ Cells Vertebrates / genetics* |
| IF | 10.806 |
| 引用数 | 278 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 49 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 9.0 |
| リソース情報 | |
| ゼブラフィッシュ | Review |