| RRC ID |
41390
|
| Author |
Itoh H, Kataoka H, Meng JY, Hamasuna R, Kitamura N, Koono M.
|
| Title |
Mouse hepatocyte growth factor activator inhibitor type 1 (HAI-1) and type 2 (HAI-2)/placental bikunin genes and their promoters.
|
| Journal |
Biochim Biophys Acta
|
| Abstract |
Hepatocyte growth factor (HGF) activator inhibitor type 1 (HAI-1) and type 2 (HAI-2) were recently discovered as specific inhibitors of HGF activator. Each of them contains two Kunitz-type serine protease inhibitor domains and a transmembrane domain, so that their overall structures are similar to each other. In this study, mouse genes encoding HAI-1 and HAI-2 were cloned by screening of a mouse genomic bacterial artificial chromosome library and by polymerase chain reaction of mouse genomic DNA, respectively. The genes (mHAI-1 and mHAI-2) were defined to consist of 11 and eight exons spanning 11 kbp and 9.5 kbp, respectively. Neither a TATA nor CAAT box was found in 5'-flanking regions of both genes and no apparent homologous portion was observed between mHAI-1 and mHAI-2 promoter regions. Promoter assay of mHAI-1 and human HAI-1 revealed that the potential binding sites of a complex of Egr-1-3 and Sp1, which was well-conserved between human (-42 to -58) and mouse (-44 to -57), might be a key portion of its transcriptional regulation to function as not only house-keeping but also early responsive genes.
|
| Volume |
1519(1-2)
|
| Pages |
92-5
|
| Published |
2001-5-28
|
| DOI |
10.1016/s0167-4781(01)00216-0
|
| PII |
S0167-4781(01)00216-0
|
| PMID |
11406276
|
| MeSH |
Animals
Base Sequence
HeLa Cells
Humans
Membrane Glycoproteins / genetics*
Mice
Molecular Sequence Data
Placenta / metabolism
Promoter Regions, Genetic*
Proteinase Inhibitory Proteins, Secretory
Serine Endopeptidases
Trypsin Inhibitor, Kunitz Soybean*
|
| IF |
3.411
|
| Times Cited |
11
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007) |
