論文 - 詳細
RRC ID | 41394 |
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著者 | Leung WK, Yu J, Ng EK, To KF, Ma PK, Lee TL, Go MY, Chung SC, Sung JJ. |
タイトル | Concurrent hypermethylation of multiple tumor-related genes in gastric carcinoma and adjacent normal tissues. |
ジャーナル | Cancer |
Abstract |
BACKGROUND:Transcriptional silencing by CpG-island hypermethylation now is believed to be an important mechanism of tumorigenesis. To date, studies on CpG-island hypermethylation in gastric carcinoma and adjacent normal tissues are few. METHODS:The authors examined 5 gastric carcinoma cell lines, 26 frozen gastric carcinoma tissues and their adjacent nontumor area for concurrent CpG-island hypermethylation in 6 tumor-related genes (p15, p16, E-cadherin, GST-pi, hMLH1, and VHL) by methylation-specific polymerase chain reaction. Nontumorous gastric tissues from 10 gastritis patients were used as controls. RESULTS:Hypermethylation was not detected in any tissue taken from gastritis patients but was identified in all 5 cell lines and in 24 (92.3%) gastric carcinoma patients. CpG-island methylation in tumor-related genes also was detected in 7 out of the 25 adjacent normal tissues from cancer patients. Hypermethylation of E-cadherin, p15, and p16 were detected more frequently than GST-pi and hMLH1, whereas aberrant methylation of VHL was not detected. Concurrent hypermethylation in 2 or more tumor-related genes was detected in 3 out of the 5 gastric carcinoma cell lines, 22 (84.6%) tumor samples, and 5 (20%) adjacent gastric tissues. Eighteen (69.2%) tumor samples showed hypermethylation in >or= 3 genes. CONCLUSIONS:The current study showed that concurrent hypermethylation of multiple tumor-related genes is detected frequently in gastric carcinoma and adjacent normal tissues. Study findings suggested that a mechanism that leads to dysregulation in CpG-island methylation is likely to be involved in the early gastric carcinogenesis process. |
巻・号 | 91(12) |
ページ | 2294-301 |
公開日 | 2001-6-15 |
PII | 10.1002/1097-0142(20010615)91:12<2294::AID-CNCR1261>3.0.CO;2-G |
PMID | 11413518 |
MeSH | Adaptor Proteins, Signal Transducing Cadherins / genetics* Carcinoma / genetics* Carrier Proteins Cell Cycle Proteins* Cyclin-Dependent Kinase Inhibitor p15 Genes, p16 / genetics* Humans Immunohistochemistry Methylation MutL Protein Homolog 1 Neoplasm Proteins / genetics* Nuclear Proteins Polymerase Chain Reaction Stomach Neoplasms / genetics* Transcription Factors / genetics* Tumor Cells, Cultured Tumor Suppressor Proteins* |
IF | 5.772 |
引用数 | 126 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 | MKN-28 MKN-45 |