Reference - Detail
| RRC ID | 41396 |
|---|---|
| Author | Kawahara M, Natsume A, Terada S, Kato K, Tsumoto K, Kumagai I, Miki M, Mahoney W, Ueda H, Nagamune T. |
| Title | Replacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor. |
| Journal | Biotechnol Bioeng |
| Abstract |
Cytokines and growth factors are indispensable for the propagation and maintenance of factor-dependent mammalian cells. However, cytokines are often so expensive that the use of factor-dependent cells for industrial applications such as protein production is often not practical. Based on our previous design of a binary hen egg lysozyme (HEL)-specific receptor composed of portions of the anti-HEL antibody and the erythropoietin receptor, a new pair of chimeric receptors having the intracellular domain of gp130 were made and transfected to an interleukin-6 (IL-6)-dependent hybridoma, 7TD1. The clone expressing the two new receptors showed clear HEL dose-dependent cell growth and monoclonal antibody production in both serum-based and serum-free media without IL-6. These results establish the feasibility of applying receptor design to tailor cells for the inexpensive induction of cell growth for the purpose of producing therapeutic products. |
| Volume | 74(5) |
| Pages | 416-23 |
| Published | 2001-9-5 |
| DOI | 10.1002/bit.1132 |
| PII | 10.1002/bit.1132 |
| PMID | 11427943 |
| MeSH | Animals Antibodies, Monoclonal / metabolism Cell Division / physiology Cell Line / cytology Cell Line / metabolism Culture Media, Serum-Free / metabolism DNA-Binding Proteins / metabolism* Efficiency Hybridomas / cytology Hybridomas / metabolism* Interleukin-6 / metabolism* Mice Muramidase / metabolism* Phosphorylation Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism* STAT3 Transcription Factor Trans-Activators / metabolism* Transfection |
| IF | 4.002 |
| Times Cited | 13 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 7-TD-1(RCB1190) |