| RRC ID |
41396
|
| Author |
Kawahara M, Natsume A, Terada S, Kato K, Tsumoto K, Kumagai I, Miki M, Mahoney W, Ueda H, Nagamune T.
|
| Title |
Replacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor.
|
| Journal |
Biotechnol Bioeng
|
| Abstract |
Cytokines and growth factors are indispensable for the propagation and maintenance of factor-dependent mammalian cells. However, cytokines are often so expensive that the use of factor-dependent cells for industrial applications such as protein production is often not practical. Based on our previous design of a binary hen egg lysozyme (HEL)-specific receptor composed of portions of the anti-HEL antibody and the erythropoietin receptor, a new pair of chimeric receptors having the intracellular domain of gp130 were made and transfected to an interleukin-6 (IL-6)-dependent hybridoma, 7TD1. The clone expressing the two new receptors showed clear HEL dose-dependent cell growth and monoclonal antibody production in both serum-based and serum-free media without IL-6. These results establish the feasibility of applying receptor design to tailor cells for the inexpensive induction of cell growth for the purpose of producing therapeutic products.
|
| Volume |
74(5)
|
| Pages |
416-23
|
| Published |
2001-9-5
|
| DOI |
10.1002/bit.1132
|
| PII |
10.1002/bit.1132
|
| PMID |
11427943
|
| MeSH |
Animals
Antibodies, Monoclonal / metabolism
Cell Division / physiology
Cell Line / cytology
Cell Line / metabolism
Culture Media, Serum-Free / metabolism
DNA-Binding Proteins / metabolism*
Efficiency
Hybridomas / cytology
Hybridomas / metabolism*
Interleukin-6 / metabolism*
Mice
Muramidase / metabolism*
Phosphorylation
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism*
STAT3 Transcription Factor
Trans-Activators / metabolism*
Transfection
|
| IF |
4.002
|
| Times Cited |
13
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
7-TD-1(RCB1190) |
