RRC ID 41407
著者 Sugawara T, Abe S, Sakuragi N, Fujimoto Y, Nomura E, Fujieda K, Saito M, Fujimoto S.
タイトル RIP 140 modulates transcription of the steroidogenic acute regulatory protein gene through interactions with both SF-1 and DAX-1.
ジャーナル Endocrinology
Abstract Coregulators have been suggested to act as a bridging apparatus between nuclear receptors and the transcriptional machinery. The orphan receptor SF-1 plays a role in controlling the basal and cAMP-stimulated expression of the human steroidogenic acute regulatory protein gene. DAX-1 is the gene responsible for X-linked adrenal hypoplasia congenita and blocks steroid biosynthesis by impairing the expression of steroidogenic acute regulatory protein. In the present study we examined the role of coregulators in the actions of SF-1 and DAX-1 on the human steroidogenic acute regulatory protein promoter. We found that the coregulator RIP 140 interacts with SF-1 in the yeast two-hybrid system. Glutathione-S-transferase pull-down assays and coimmunoprecipitations confirmed the interaction between RIP 140 and SF-1. RIP 140 was also shown to interact with DAX-1. When an RIP 140 expression vector was introduced into Y-1 cells, basal and cAMP-stimulated human steroidogenic acute regulatory protein promoter activities decreased. The inhibitory effect of RIP 140 on human steroidogenic acute regulatory protein promoter activity was dependent upon the presence of SF-1. The cAMP response of an SF-1 response element was inhibited by both RIP 140 and DAX-1 expression vectors at low concentrations of plasmids. We conclude that RIP 140 binds to the orphan nuclear receptor SF-1 and DAX-1 and modulates their actions on the human steroidogenic acute regulatory protein promoter.
巻・号 142(8)
ページ 3570-7
公開日 2001-8-1
DOI 10.1210/endo.142.8.8309
PMID 11459805
MeSH Adaptor Proteins, Signal Transducing Animals COS Cells Cricetinae DAX-1 Orphan Nuclear Receptor DNA-Binding Proteins / physiology* Fushi Tarazu Transcription Factors Homeodomain Proteins Humans Mice Nuclear Proteins / physiology* Nuclear Receptor Interacting Protein 1 Phosphoproteins / genetics* Promoter Regions, Genetic / physiology Receptors, Cytoplasmic and Nuclear Receptors, Retinoic Acid / physiology* Repressor Proteins* Steroidogenic Factor 1 Transcription Factors / physiology* Transcription, Genetic / physiology* Tumor Cells, Cultured
IF 3.934
引用数 38
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
ヒト・動物細胞 Y1(RCB0533) COS-1(RCB0143)