論文 - 詳細
| RRC ID | 41408 |
|---|---|
| 著者 | Sato K, Sasaki K, Akiyama Y, Yamaguchi K. |
| タイトル | Mass spectrometric high-throughput analysis of serum-free conditioned medium from cancer cell lines. |
| ジャーナル | Cancer Lett |
| Abstract |
Proteins secreted by cancer cells may be useful as tumor markers. We analyzed constituents produced by cells grown in serum-free conditioned medium to avoid confounding factors present in serum. Mass spectrometric techniques were used to obtain expression patterns of constituents between 2000 and 15000 Da from as little as 1 microl of crude sample. This protocol can produce distinctive mass profiles from 16 cell lines within 1 h. Thus, differential display by mass spectrometry will expedite the discovery of peptides specifically secreted by particular cells. These data illustrate the advantage of this strategy over the conventional approach of electrophoretic separation of serum samples. |
| 巻・号 | 170(2) |
| ページ | 153-9 |
| 公開日 | 2001-9-20 |
| DOI | 10.1016/s0304-3835(01)00567-5 |
| PII | S0304383501005675 |
| PMID | 11463493 |
| MeSH | Culture Media, Conditioned / analysis Culture Media, Serum-Free / analysis* Electrophoresis, Polyacrylamide Gel Glycine / analogs & derivatives Glycine / chemistry Humans Mass Spectrometry / methods* Molecular Weight Peptide Mapping Tumor Cells, Cultured / chemistry* |
| IF | 7.36 |
| 引用数 | 14 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN7(RCB0999) MKN74(RCB1002) MKN1(RCB1003) |