RRC ID 41415
著者 Tamura S, Kataoka H, Matsui Y, Shionoya Y, Ohno K, Michi KI, Takahashi K, Yamaguchi A.
タイトル The effects of transplantation of osteoblastic cells with bone morphogenetic protein (BMP)/carrier complex on bone repair.
ジャーナル Bone
Abstract We investigated the effects of transplantation of osteoblastic cells with a bone morphogenetic protein (BMP)/carrier complex on bone repair by in vitro and in vivo experiments. Poly-D,L-lactic-co-glycolic acid/gelatin sponge (PGS) was used as a carrier for cell transplantation. In the in vitro experiments, three cell types, C3H10T1/2 cells, MC3T3-E1 cells, and primary osteoblastic cells, isolated from newborn rat calvariae (ROB cells), were cultured for 2 weeks on PGS alone or PGS containing BMP-2 (PGS/BMP). C3H10T1/2 cells cultured on PGS/BMP expressed several markers related to differentiation of both osteoblasts and chondrocytes, such as alkaline phosphatase (ALP) activity and mRNAs for osteocalcin and aggrecan, whereas the cells cultured on PGS alone expressed no such markers. MC3T3-E1 cells cultured on PGS/BMP exhibited a more ALP-positive cells than those cultured on PGS alone. PGS/BMP promoted ROB cell differentiation into both osteoblasts and chondrocytes. In the in vivo experiments, we transplanted ROB cells, which had been cultured on PGS alone or PGS/BMP in vitro for 2 weeks, into bone defects created in rat calvariae. Transplantation of ROB cells cultured on PGS alone generated little new bone. Transplantation of ROB cells cultured on PGS, which absorbed a low dose (10 ng) of rhBMP-2,; induced significantly higher bone mineral content than PGS/BMP alone, although application of a high dose (1 microg) of rhBMP-2 induced no difference in bone mineral content between transplantation of PGS/BMP with or without ROB cells. These results show that transplantation of osteoblastic cells after induction of osteoblast maturation in vitro by cultivation on PGS/BMP is a potent technique for cell therapy of bone repair.
巻・号 29(2)
ページ 169-75
公開日 2001-8-1
DOI 10.1016/s8756-3282(01)00498-7
PII S8756-3282(01)00498-7
PMID 11502479
MeSH Aggrecans Animals Base Sequence Bone Morphogenetic Proteins / metabolism* Bone Remodeling* Carrier Proteins / metabolism* Cell Line Cell Transplantation* DNA Primers Extracellular Matrix Proteins* Lectins, C-Type Mice Osteoblasts / cytology* Osteoblasts / metabolism Osteocalcin / genetics Proteoglycans / genetics RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction
IF 4.147
引用数 33
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126) 10T1/2(RCB0247)