論文 - 詳細
| RRC ID | 41416 |
|---|---|
| 著者 | Kitajima H, Okubo Y, Honda J, Yonemitsu J, Yoshida N, Fumimori T, Oizumi K. |
| タイトル | Interleukin-4 is needed for the infection of monocytes by human cytomegalovirus. |
| ジャーナル | Intervirology |
| Abstract |
OBJECTIVE:We studied the ability of human cytomegalovirus (HCMV) to infect peripheral blood mononuclear cells (PBMC) pretreated with or without Th2-cytokine interleukin-4 (IL-4) in vitro. METHODS:Adherent cells and nonadherent cells were obtained from PBMC. We inoculated these cells with HCMV at concentrations ranging from 0 to 10 ng/ml of IL-4. Immediate-early antigen-1 (IE-1) and glycoprotein H (gH) mRNAs were detected using the reverse-transcription polymerase chain reaction. RESULTS:IE-1 and gH mRNAs could be detected in monocytes pretreated with IL-4. In contrast, no IE-1 mRNA was detected in monocytes pretreated without IL-4. We tested whether higher infectious titers could result in the infection of monocytes whether or not they were pretreated with IL-4. However, no IE-1 mRNA was detected in the monocytes not pretreated with IL-4. To elucidate how HCMV-infected monocytes affect lung tissue, human embryonic lung fibroblasts MRC-5 were cocultured with HCMV-infected monocytes. The cytopathic effects of HCMV were observed microscopically and was confirmed by direct immunoperoxidase staining with a human monoclonal antibody against the HCMV IE-1. CONCLUSION:Our data strongly suggest that the ability of HCMV to infect monocytes may correlate with the presence of IL-4. |
| 巻・号 | 44(4) |
| ページ | 264-70 |
| 公開日 | 2001-1-1 |
| DOI | 10.1159/000050057 |
| PII | 50057 |
| PMID | 11509890 |
| MeSH | Cytomegalovirus / drug effects Cytomegalovirus / immunology* Cytomegalovirus Infections / immunology Humans Immediate-Early Proteins / biosynthesis Immediate-Early Proteins / genetics Interleukin-4 / pharmacology* Monocytes / drug effects Monocytes / immunology* RNA, Messenger / analysis Recombinant Proteins / pharmacology Viral Envelope Proteins / biosynthesis Viral Envelope Proteins / genetics Virus Replication |
| IF | 1.235 |
| 引用数 | 3 |
| WOS 分野 | VIROLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MRC-5 |