論文 - 詳細
| RRC ID | 41422 |
|---|---|
| 著者 | Seo N, Hayakawa S, Takigawa M, Tokura Y. |
| タイトル | Interleukin-10 expressed at early tumour sites induces subsequent generation of CD4(+) T-regulatory cells and systemic collapse of antitumour immunity. |
| ジャーナル | Immunology |
| Abstract |
We investigated the relationship between transforming growth factor-beta (TGF-beta)-secreting T-regulatory (Tr) cells and anti-B16 melanoma immunity, and studied the association of early cytokines expressed at tumour sites with the generation of Tr cells. A large number of CD4(+) Tr cells producing interleukin (IL)-4, IL-10 and TGF-beta accumulated with functionally depressed CD8(+) cytotoxic T lymphocytes (CTLs) at tumour sites on day 20 after subcutaneous (s.c.) inoculation of B16 tumour cells. Tr cells consisted of two populations, which were termed T helper 3 (Th3) and Tr1 cells. B16-infiltrating Tr cells strongly inhibited the generation of B16-specific T helper 1 (Th1) cells in a TGF-beta-dependent manner and were assumed to suppress effective generation of CTLs. In addition, B16 cells markedly progressed in mice transferred adoptively by the cultured B16-infiltrating Tr cells compared with untreated mice. The capacity of these Tr cells to produce TGF-beta was hampered by neutralizing anti-IL-10 and partly anti-IL-4 monoclonal antibodies (mAbs) injected intralesionally during the early development of B16 tumours, and this treatment markedly attenuated B16 growth. Furthermore, a lesional injection of recombinant mouse IL-10 at an early tumour site resulted in the vigorous progression of B16 tumours. These results provide evidence that Tr cells, belonging to the T helper 3/T-regulatory 1 (Th3/Tr1) type, are activated in B16-bearing hosts under the influence of T helper 2 (Th2) cytokines, mainly IL-10 (produced at early tumour lesions), and that this regulatory T-cell population functions as a suppressor of anti-B16 immunity. |
| 巻・号 | 103(4) |
| ページ | 449-57 |
| 公開日 | 2001-8-1 |
| DOI | 10.1046/j.1365-2567.2001.01279.x |
| PII | imm1279 |
| PMID | 11529935 |
| PMC | PMC1783257 |
| MeSH | Animals CD4-Positive T-Lymphocytes / immunology* Cell Culture Techniques Disease Progression Down-Regulation / immunology Female Immune Tolerance* Immunity, Cellular Interleukin-10 / immunology* Interleukin-10 / metabolism Lymphocytes, Tumor-Infiltrating / immunology* Melanoma, Experimental / immunology* Mice Mice, Inbred C57BL T-Lymphocyte Subsets / immunology Transforming Growth Factor beta / metabolism |
| IF | 5.016 |
| 引用数 | 152 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16 melanoma 4A5(RCB0557) |