論文 - 詳細
| RRC ID | 41423 |
|---|---|
| 著者 | Wong BC, Wang WP, Cho CH, Fan XM, Lin MC, Kung HF, Lam SK. |
| タイトル | 12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells. |
| ジャーナル | Carcinogenesis |
| Abstract |
Arachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution. |
| 巻・号 | 22(9) |
| ページ | 1349-54 |
| 公開日 | 2001-9-1 |
| DOI | 10.1093/carcin/22.9.1349 |
| PMID | 11532854 |
| MeSH | 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / pharmacology Apoptosis / drug effects* Apoptosis / physiology Arachidonate 12-Lipoxygenase / biosynthesis Arachidonate 12-Lipoxygenase / genetics Blood Platelets / enzymology Blotting, Western Caspase 3 Caspase 7 Caspases / metabolism Cell Division / drug effects Cell Division / physiology Drug Interactions Flavanones* Flavonoids / pharmacology* Gene Expression Regulation, Neoplastic / drug effects Growth Inhibitors / pharmacology Humans Lipoxygenase Inhibitors* / pharmacology* RNA, Messenger / biosynthesis RNA, Messenger / genetics Stomach Neoplasms / enzymology Stomach Neoplasms / pathology* Tumor Cells, Cultured |
| IF | 4.603 |
| 引用数 | 86 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MKN28(RCB1000) |