| RRC ID |
41442
|
| 著者 |
Hayashi M, Tomita M, Yoshizato K.
|
| タイトル |
Production of EGF-collagen chimeric protein which shows the mitogenic activity.
|
| ジャーナル |
Biochim Biophys Acta
|
| Abstract |
Collagen has been utilized as a natural biomaterial because of its high biocompatibility, adhesiveness to cells and tissues, and biodegradability. The present study developed a recombinant technology to confer a mitogenic activity on type III collagen by fusing it to epidermal growth factor (EGF) at the collagen's N-terminus. The chimeric protein of EGF-collagen was synthesized in insect cells by the baculovirus-insect cell expression system. The fusion protein was shown to hold the triple helical conformation of collagen and the mitogenic activity of EGF. It was also demonstrated that the chimeric protein can be immobilized on tissue culture dishes as a fibrous form and in collagen fibrils without abolishing the original mitogenic activity of EGF. This fusion protein can be utilized as a biocompatible, biodegradable, and adhesive fibrous mitogen for a variety of purposes in the area of tissue engineering.
|
| 巻・号 |
1528(2-3)
|
| ページ |
187-95
|
| 公開日 |
2001-10-3
|
| DOI |
10.1016/s0304-4165(01)00187-8
|
| PII |
S0304416501001878
|
| PMID |
11687306
|
| MeSH |
Animals
Baculoviridae / metabolism
Cell Line
Collagen / chemistry*
Culture Media
Epidermal Growth Factor / chemistry*
Gels
Genetic Vectors
Mitogens / biosynthesis*
Recombinant Fusion Proteins / biosynthesis*
|
| IF |
3.411
|
| 引用数 |
19
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Sf9(RCB0563) |
